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High-throughput collagen fingerprinting of intact microfaunal remains; a low-cost method for distinguishing between murine rodent bones.
Buckley M, et al.
Rapid Communications in Mass Spectrometry, 30, 805-812 (2016)
Pingbo Zhang et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(26), 11829-11834 (2010-06-16)
Phosphatidylinositol (3,4,5)-triphosphate (PtdInsP(3)) mediates intracellular signaling for directional sensing and pseudopod extension at the leading edge of migrating cells during chemotaxis. How this PtdInsP(3) signal is translated into remodeling of the actin cytoskeleton is poorly understood. Here, using a proteomics
Shu-Quan Fan et al.
The Journal of biological chemistry, 287(14), 11272-11281 (2012-02-10)
Endo-β-N-acetylglucosaminidase from Streptococcus pneumoniae (Endo-D) is an endoglycosidase capable of hydrolyzing the Fc N-glycan of intact IgG antibodies after sequential removal of the sialic acid, galactose, and internal GlcNAc residues in the N-glycan. Endo-D also possesses transglycosylation activity with sugar
K de la Luz-Hernández et al.
Vaccine, 32(24), 2851-2858 (2014-03-20)
The development of safe, effective, and affordable vaccines has become a global effort due to its vast impact on overall world health conditions. A brief overview of vaccine characterization techniques, especially in the area of high-resolution mass spectrometry, is presented.
Wei Huang et al.
Organic & biomolecular chemistry, 8(22), 5224-5233 (2010-09-18)
CD52 is a glycosylphosphatidylinositol (GPI)-anchored glycopeptide antigen found on sperm cells and human lymphocytes. Recent structural studies indicate that sperm-associated CD52 antigen carries both a complex type N-glycan and an O-glycan on the polypeptide backbone. To facilitate functional and immunological
Paula Cabrita et al.
Talanta, 83(2), 457-463 (2010-11-30)
Four strains of Listeria monocytogenes with different levels of virulence were studied. Two strains were consistently evaluated as virulent (strain 3077) and of low virulence (strain 3993), whereas the other two strains (3006 and 3049) originated conflicting results in what
Thibault Chaze et al.
Molecular & cellular proteomics : MCP, 12(2), 283-301 (2012-11-14)
Exposure of the skin to ionizing radiation leads to characteristic reactions that will often turn into a pathophysiological process called the cutaneous radiation syndrome. The study of this disorder is crucial to finding diagnostic and prognostic bioindicators of local radiation
J Almeida et al.
Metabolic brain disease, 34(1), 141-152 (2018-10-12)
Gliomas represent the most common primary malignant brain tumors in adults, with an extremely poor prognosis. Among several risk factors, lifestyle was also recently identified as a major risk factor for the development of primary glioma. In the present study
Wei Huang et al.
Journal of the American Chemical Society, 131(49), 17963-17971 (2009-11-18)
N-Glycans are major components of many glycoproteins. These sugar moieties are frequently involved in important physiological and disease processes via their interactions with a variety of glycan-binding proteins (GBP). Clustering effect is an important feature in many glycan-lectin interactions. We
Helen Wang et al.
Bioorganic & medicinal chemistry, 21(7), 2037-2044 (2013-02-16)
We report a facile synthesis of glycoprotein-based glyco-ligands and their binding with influenza hemagglutinin and human galectin-3. Human serum albumin (HSA) was used as the scaffold and an Asn-linked complex type N-glycan prepared from chicken eggs was used as the
An improved clean sonoreactor-based method for protein identification by mass spectrometry-based techniques.
Santos HM, et al.
Talanta, 77, 870-875 (2008)
Sunghyun Kang et al.
The Journal of biological chemistry, 280(45), 37698-37706 (2005-09-09)
Transcriptional signaling from the Ca(2+)-calmodulin-activated phosphatase calcineurin to its substrate NFAT (nuclear factor of activated T cells, also termed NFATc) is critically dependent on a protein-protein docking interaction between calcineurin and the PXIXIT motif in NFAT. Several inhibitors of NFAT-calcineurin
R Rial-Otero et al.
Journal of proteome research, 6(2), 909-912 (2007-02-03)
Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the adenylylsulphate
Guillaume Bastin et al.
Frontiers in microbiology, 11, 1157-1157 (2020-06-26)
Pathogenic enteric viruses and bacteriophages such as Qβ and MS2 are transmitted through the fecal-oral route. However, oxidants such as peroxynitrite (ONOOH) and hypochlorous acid (HClO) can prevent new infection by inactivating infectious viruses. Their virucidal effect is well recognized
Design and synthesis of glycoprotein-based multivalent glyco-ligands for influenza hemagglutinin and human galectin-3.
Wang, H., et al.
Bioorganic & Medicinal Chemistry Letters, 21, 2037-2044 (2013)
Juan Pablo Maianti et al.
Nature chemical biology, 15(6), 565-574 (2019-05-16)
Enzymes that act on multiple substrates are common in biology but pose unique challenges as therapeutic targets. The metalloprotease insulin-degrading enzyme (IDE) modulates blood glucose levels by cleaving insulin, a hormone that promotes glucose clearance. However, IDE also degrades glucagon
Anna Swierzko et al.
Glycobiology, 22(2), 267-280 (2011-09-06)
Ficolin-1 (M), ficolin-2 (L), ficolin-3 (H) and mannan-binding lectin (MBL) activate the complement system and have opsonic activity. The specificity of ficolin-3 is poorly characterized and currently limited to a few ligands only. We present new specific targets for human
Ultrasonic energy as a new tool for fast isotopic 18O labeling of proteins for mass spectrometry-based techniques: preliminary results.
Carreira RJ, et al.
Talanta, 76, 400-406 (2008)
Wei Huang et al.
Journal of the American Chemical Society, 134(29), 12308-12318 (2012-07-04)
The fine structures of Fc N-glycans can modulate the effector functions of IgG antibodies. It has been demonstrated that lack of the core fucose on the Fc N-glycans leads to drastic enhancement of antibody-dependent cellular cytotoxicity (ADCC), while terminal α2,6-sialylation
Wei Huang et al.
Journal of the American Chemical Society, 131(6), 2214-2223 (2009-02-10)
Homogeneous N-glycoproteins carrying defined natural N-glycans are essential for detailed structural and functional studies. The transglycosylation activity of the endo-beta-N-acetylglucosaminidases from Arthrobacter protophormiae (Endo-A) and Mucor hiemalis (Endo-M) holds great potential for glycoprotein synthesis, but the wild-type enzymes are not
Yadong Wei et al.
Biochemistry, 47(39), 10294-10304 (2008-09-06)
The presence and precise structures of the glycans attached at the Fc domain of monoclonal antibodies play an important role in determining antibodies' effector functions such as antibody-dependent cell cytotoxicity (ADCC), complement activation, and anti-inflammatory activity. This paper describes a
Mike Buckley et al.
Rapid communications in mass spectrometry : RCM, 30(7), 805-812 (2016-07-14)
Microfaunal skeletal remains can be sensitive indicators of the contemporary ecosystem in which they are sampled and are often recovered in owl pellets in large numbers. Species identification of these remains can be obtained using a range of morphological criteria
Cindy Loui et al.
BMC microbiology, 9, 183-183 (2009-09-01)
The global regulatory system ArcAB controls the anaerobic growth of E. coli, however, its role in aerobic conditions is not well characterized. We have previously reported that ArcA was necessary for Salmonella to resist reactive oxygen species (ROS) in aerobic
Wei Huang et al.
Chembiochem : a European journal of chemical biology, 12(6), 932-941 (2011-03-05)
Structurally well defined, homogeneous glycopeptides and glycoproteins are indispensable tools for functional glycomics studies. By screening of various endo-β-N-acetylglucosaminidases through the use of appropriate synthetic donor and acceptor substrates, we have found that the Flavobacterium meningosepticum endo-β-N-acetyl-glucosaminidases (GH family 18)
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