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" no prazo de Papéis
Amanda J MacFarlane et al.
The Journal of nutrition, 144(4), 419-424 (2014-02-07)
Folate metabolism affects DNA synthesis, methylation, mutation rates, genomic stability, and gene expression, which are altered in colon cancer. Serine hydroxymethyltransferase 1 (SHMT1) regulates thymidylate (dTMP) biosynthesis and uracil accumulation in DNA, and as such affects genome stability. Previously, we
Gui-Ying Mei et al.
International journal of food microbiology, 215, 7-15 (2015-09-01)
Understanding the survival mechanisms used by Shiga toxin-producing Escherichia coli (STEC), including O157:H7 and non-O157 serotypes, is important for minimizing contamination of fresh produce and occurrence of foodborne outbreaks. Recent outbreaks linked to leafy green vegetables and sprouted seeds have
Osamu Totsuka et al.
Gan to kagaku ryoho. Cancer & chemotherapy, 40(13), 2525-2528 (2013-12-18)
We have reported, in a randomized, controlled study, that tegafur-uracil(UFT)and protein-bound polysaccharide K(PSK)combination therapy significantly improves the 5-year disease-free survival rate and reduces the risk of recurrence compared to UFT alone for Stage II or III colorectal cancer. In this
Kyoung-Hwa Choi et al.
Extremophiles : life under extreme conditions, 19(5), 909-920 (2015-06-25)
Sulfolobus acidocaldarius DSM639 produced an acid-resistant membrane-bound amylopullulanase (Apu) during growth on starch as a sole carbon and energy source. The physiological role of Apu in starch metabolism was investigated by the growth and starch degradation pattern of apu disruption
Hiroshi Kikukawa et al.
Journal of biotechnology, 208, 63-69 (2015-06-09)
The oil-producing zygomycete Mortierella alpina 1S-4 is known to accumulate beneficial polyunsaturated fatty acids. We identified the lig4 gene that encodes for a DNA ligase 4 homolog, which functions to repair double strand breaks by non-homologous end joining. We disrupted
Simon Forster et al.
Journal of chromatography. A, 1283, 110-115 (2013-02-26)
The unquestioned potential of open tubular column design for miniaturized liquid chromatography systems has been assessed recently. Instrument limitation and insufficient mass loadability of the columns, however, have prevented experimental breakthrough in this field, the latter requiring new methods for
Mika Nabeno et al.
Biochemical and biophysical research communications, 434(2), 191-196 (2013-03-19)
In recent years, various dipeptidyl peptidase IV (DPP-4) inhibitors have been released as therapeutic drugs for type 2 diabetes in many countries. In spite of their diverse chemical structures, no comparative studies of their binding modes in the active site
K Ichinohe et al.
Annals of the Royal College of Surgeons of England, 95(2), e52-e54 (2013-03-15)
Primary retroperitoneal transitional cell carcinomas (TCCs) are extremely rare neoplasms for which prognosis is very poor. We present a case that underwent complete remission after radiotherapy and concurrent oral chemotherapy. A 68-year-old woman presented with acute onset of bloody stool.
Diane E Brown et al.
Infection and immunity, 83(6), 2290-2299 (2015-04-01)
The Gram-negative intracellular bacterium Salmonella enterica serovar Typhimurium causes persistent systemic inflammatory disease in immunocompetent mice. Following oral inoculation with S. Typhimurium, mice develop a hematopathological syndrome akin to typhoid fever with splenomegaly, microcytic anemia, extramedullary erythropoiesis, and increased hemophagocytic
Stephen R Groskreutz et al.
Journal of chromatography. A, 1405, 133-139 (2015-06-21)
On-column focusing is essential for satisfactory performance using capillary scale columns. On-column focusing results from generating transient conditions at the head of the column that lead to high solute retention. Solvent-based on-column focusing is a well-known approach to achieve this.
Tadashi Miyazawa et al.
Chemical communications (Cambridge, England), 49(71), 7851-7853 (2013-07-31)
The novel 1,2-dithianenucleoside was designed as a hybrid type of modification between 4'-thioribonucleoside and altritol nucleoside. The desired compound, i.e., 1-[(3R,4R,5S,6R)-4,5-dihydroxy-6-hydroxymethyl-1,2-dithianyl]uracil (20), was prepared via the Pummerer-like reaction, followed by Vorbruggen glycosylation between an appropriately protected 1,2-dithiane derivative and silylated
J W Cary et al.
Eukaryotic cell, 14(10), 983-997 (2015-07-26)
The global regulatory veA gene governs development and secondary metabolism in numerous fungal species, including Aspergillus flavus. This is especially relevant since A. flavus infects crops of agricultural importance worldwide, contaminating them with potent mycotoxins. The most well-known are aflatoxins
Hana Vlčková et al.
Journal of separation science, 37(11), 1297-1307 (2014-03-22)
The retention behavior of a large group of analytes (35) with varied properties (pKa and logP) was studied on eight hydrophilic interaction LC columns with different surfaces, stationary phase chemistries, and types of particles. The acetonitrile content (5-95%), buffer concentration
Amy I Yu et al.
Cell reports, 31(1), 107471-107471 (2020-04-09)
There is increasing evidence that gut microbiome perturbations, also known as dysbiosis, can influence colorectal cancer development. To understand the mechanisms by which the gut microbiome modulates cancer susceptibility, we examine two wild-type mouse colonies with distinct gut microbial communities
Go Kagiya et al.
Oncology reports, 34(2), 1065-1073 (2015-06-03)
The presence of hypoxic regions within solid tumors is caused by an imbalance between cell proliferation and angiogenesis. Such regions may facilitate the onset of recurrence after radiation therapy and chemotherapy, as hypoxic cells show resistance to these treatments. We
Joseph D Schonhoft et al.
Biochemistry, 52(15), 2536-2544 (2013-03-20)
Human uracil DNA glycosylase (hUNG) plays a central role in DNA repair and programmed mutagenesis of Ig genes, requiring it to act on sparsely or densely spaced uracil bases located in a variety of contexts, including U/A and U/G base
Abul K Mallik et al.
Analytical and bioanalytical chemistry, 406(19), 4585-4593 (2014-05-27)
A new hydrophilic and nonionic poly(2-vinyloxazoline)-grafted silica (Sil-VOX(n)) phase was synthesized and applied for the separation of nucleosides and nucleobases in hydrophilic interaction chromatography (HILIC). Polymerization and immobilization onto silica were confirmed by using characterization techniques including (1)H NMR spectroscopy
Radika Soysa et al.
The Journal of biological chemistry, 288(41), 29954-29964 (2013-08-30)
The pathogenic protozoan parasite Leishmania donovani is capable of both de novo pyrimidine biosynthesis and salvage of pyrimidines from the host milieu. Genetic analysis has authenticated L. donovani uracil phosphoribosyltransferase (LdUPRT), an enzyme not found in mammalian cells, as the
Paulina Prorok et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), E3695-E3703 (2013-09-12)
Spontaneous hydrolytic deamination of cytosine to uracil (U) in DNA is a constant source of genome instability in cells. This mutagenic process is greatly enhanced at high temperatures and in single-stranded DNA. If not repaired, these uracil residues give rise
A Marechal et al.
Journal of chromatography. A, 1365, 140-147 (2014-09-25)
This paper presents a photografting process of monolithic silica capillary columns based on the ene-thiol click chemistry. This study is performed on a "generic" vinyl-functionalized silica monolith (Hmin 6±1μm). The photoclick reaction is investigated using different thiol monomers (octadecanethiol, cysteine
Yuji Toiyama et al.
Anticancer research, 33(11), 5065-5074 (2013-11-14)
The clinical significance of the systemic inflammatory response (SIR) in patients with rectal cancer undergoing neoadjuvant chemoradiotherapy (CRT), to the best of our knowledge, has not been thus far investigated. The neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR) and C-Reactive protein
Gurbet Karahan et al.
Oncology reports, 33(6), 3131-3145 (2015-05-13)
Ribosomal RNA (rRNA) expression, one of the most important factors regulating ribosome production, is primarily controlled by a CG-rich 45 S rDNA promoter. However, the DNA methylation state of the 45 S rDNA promoter, as well as its effect on
Bernard Charbonnel et al.
Hospital practice (1995), 41(2), 93-107 (2013-05-18)
As type 2 diabetes mellitus (T2DM) progresses, most patients will require insulin replacement therapy. Whether oral antidiabetic drug (OAD) therapy should be retained when initiating insulin is still debated. While the rationale to keep metformin with insulin is strong (mostly
Xiaoli Xue et al.
ACS synthetic biology, 4(6), 700-706 (2014-12-11)
Top-down reduction of the bacterial genome to construct desired chassis cells is important for synthetic biology. However, the current progress in the field of genome reduction is greatly hindered by indispensable life-essential genes that are interspersed throughout the chromosomal loci.
Heidi Redden et al.
Nature communications, 6, 7810-7810 (2015-07-18)
Synthetic promoters, especially minimally sized, are critical for advancing fungal synthetic biology. Fungal promoters often span hundreds of base pairs, nearly ten times the amount of bacterial counterparts. This size limits large-scale synthetic biology efforts in yeasts. Here we address
Kazufumi Mochizuki et al.
Biochemical and biophysical research communications, 436(3), 497-502 (2013-06-19)
The various properties of small RNAs, such as length, terminal nucleotide, thermodynamic asymmetry and duplex mismatches, can impact their sorting into different Argonaute proteins in diverse eukaryotes. The developmentally regulated 26- to 32-nt siRNAs (scnRNAs) are loaded to the Argonaute
Ryszard Olinski et al.
Mutation research, 705(3), 239-245 (2010-08-17)
Uracil may arise in DNA as a result of spontaneous cytosine deamination and/or misincorporation of dUMP during DNA replication. In this paper we will review: (i) sources of the origin of uracil in DNA; (ii) some properties of the enzymes
Er-Ping Liu et al.
Genetic testing and molecular biomarkers, 19(11), 610-616 (2015-09-12)
Point-of-care genetic analysis may require polymerase chain reaction (PCR) to be carried out on whole blood. However, human blood contains natural inhibitors of PCR such as hemoglobin, immunoglobulin G, lactoferrin, and proteases, as well as anticoagulant agents, including EDTA and
Dipeptidyl peptidase-4 inhibitors and cardiovascular safety.
Timothy M E Davis
The Medical journal of Australia, 200(8), 450-451 (2014-05-06)
Rasime Kalkan et al.
Gene, 554(1), 81-86 (2014-12-03)
To establish the frequency of IDH1 mutations and MGMT methylation in primary glioblastomas. We screened primary glioblastoma multiforme (GBM) in a population-based study for IDH1 mutations and MGMT methylation and correlated them with clinical data. IDH1 mutations were detected in
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