Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes.

Nucleic acids research (1995-08-25)
K Tang, D Fu, S Kötter, R J Cotter, C R Cantor, H Köster

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.

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3-Hydroxypicolinic acid, matrix substance for MALDI-MS, ≥99.0% (HPLC)