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Plasma Membrane Domain Patterning and Self-Reinforcing Polarity in Arabidopsis.

Developmental cell (2019-12-24)
Petra Marhava, Ana Cecilia Aliaga Fandino, Samuel W H Koh, Adriana Jelínková, Martina Kolb, Dorina P Janacek, Alice S Breda, Pietro Cattaneo, Ulrich Z Hammes, Jan Petrášek, Christian S Hardtke

Cell polarity is a key feature in the development of multicellular organisms. For instance, asymmetrically localized plasma-membrane-integral PIN-FORMED (PIN) proteins direct transcellular fluxes of the phytohormone auxin that govern plant development. Fine-tuned auxin flux is important for root protophloem sieve element differentiation and requires the interacting plasma-membrane-associated BREVIS RADIX (BRX) and PROTEIN KINASE ASSOCIATED WITH BRX (PAX) proteins. We observed "donut-like" polar PIN localization in developing sieve elements that depends on complementary, "muffin-like" polar localization of BRX and PAX. Plasma membrane association and polarity of PAX, and indirectly BRX, largely depends on phosphatidylinositol-4,5-bisphosphate. Consistently, mutants in phosphatidylinositol-4-phosphate 5-kinases (PIP5Ks) display protophloem differentiation defects similar to brx mutants. The same PIP5Ks are in complex with BRX and display "muffin-like" polar localization. Our data suggest that the BRX-PAX module recruits PIP5Ks to reinforce PAX polarity and thereby the polarity of all three proteins, which is required to maintain a local PIN minimum.

Product Number
Product Description

2,4-Dichlorophenoxyacetic acid, ≥95%, crystalline
Propidium iodide solution, solution (1.0 mg/ml in water)
Bikinin, ≥98% (HPLC)
Anti-GFP, from mouse IgG1κ (clones 7.1 and 13.1)
cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack Protease Inhibitor Cocktail, Tablets supplied in foil blister packs.
Driselase from Basidiomycetes sp., powder, Protein ≥10 % by biuret
Kinetin, suitable for plant cell culture, crystalline
Paraformaldehyde, powder, 95%
Magnesium sulfate heptahydrate, puriss. p.a., ACS reagent, ≥99.0% (KT)