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Primary Olfactory Ensheathing Cell Culture from Human Olfactory Mucosa Specimen.

Bio-protocol (2017-05-20)
Mansoureh Hashemi, Mahmoudreza Hadjighassem
ABSTRACT

The human olfactory mucosa is located in the middle and superior turbinates, and the septum of nasal cavity. Olfactory mucosa plays an important role in detection of odours and it is also the only nervous tissue that is exposed to the external environment. This property leads to easy access to the olfactory mucosa for achieving various researches. The lamina propria of olfactory mucosa consists of olfactory ensheathing cells (OECs) that cover the nerve fibers of olfactory. Here we describe a protocol for isolation of OECs from biopsy of human olfactory mucosa.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Triton X-100, for molecular biology
Sigma-Aldrich
Dispase® II, protease
Sigma-Aldrich
Nerve Growth Factor-7S from murine submaxillary gland, NGF-7S, lyophilized powder, suitable for cell culture
Sigma-Aldrich
Bovine Serum Albumin, lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture
Sigma-Aldrich
Collagenase from Clostridium histolyticum, Type IA, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid, For general use
Sigma-Aldrich
Anti-S-100 antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Paraformaldehyde, reagent grade, crystalline