Merck

Separation of lipid classes by HPLC on a cyanopropyl column.

Lipids (2011-11-22)
Petter Olsson, Jan Holmbäck, Bengt Herslöf
ABSTRACT

A new method for the separation and identification of lipid classes by normal-phase HPLC on a cyanopropyl column is described. The use of a simple binary gradient, with toluene as a component, provided a rapid separation of non-polar as well as phospholipid classes. The inherent small differences in performances between possible non-polar eluent components of the gradient, such as hexane, heptane, and iso-octane, had a pronounced impact on retention times for individual phospholipid classes. Separation of molecular species within a lipid class could also be observed.

MATERIALS
Product Number
Brand
Product Description

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Density Standard 692 kg/m3, H&D Fitzgerald Ltd. Quality
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