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  • Alteration of α-tocopherol-associated protein (TAP) expression in human breast epithelial cells during breast cancer development.

Alteration of α-tocopherol-associated protein (TAP) expression in human breast epithelial cells during breast cancer development.

Food chemistry (2013-02-16)
Ka-Wai Tam, Chi-Tang Ho, Wen-Jui Lee, Shih-Hsin Tu, Ching-Shui Huang, Ching-Shyang Chen, Chia-Hwa Lee, Chih-Hsiung Wu, Yuan-Soon Ho
ABSTRACT

Breast cancer is the most common malignancy among women and has an age-specific incidence profile. Over the last decade, many studies have demonstrated the anticancer activity of α-tocopherol, the main and most active form of natural vitamin E. α-Tocopherol-associated protein (TAP) was found to be one of the major α-tocopherol binding proteins in human serum and in liver, brain, and prostate tissues. In this study, we found that reduced TAP expression was significantly correlated with Her2/neu receptor expression, breast cancer stage and nodal stage in paired normal and cancerous breast tissue samples from 93 patients using real-time PCR analysis. A cell viability assay showed that α-tocopheryl succinate (α-TOS), a synthetic derivative of α-tocopherol, enhanced the cells' sensitivity to doxorubicin and resulted in a reduction in cell viability in breast cancers. Taken together, these data suggest that the use of vitamin E or its analogue as a dietary supplement may be beneficial for the treatment of cancer.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(+)-α-Tocopherol, from vegetable oil, Type V, ~1000 IU/g
Sigma-Aldrich
DL-α-Tocopherol acetate, ≥96% (HPLC)
Sigma-Aldrich
(+)-α-Tocopherol, Type VI, from vegetable oil, liquid (≥0.88M based on potency, density and molecular wt.), BioReagent, suitable for insect cell culture, ≥1000 IU/g
Sigma-Aldrich
DL-α-Tocopherol acetate, tested according to Ph. Eur.
Supelco
Tocopheryl Acetate, a, Pharmaceutical Secondary Standard; Certified Reference Material
α-Tocopherol acetate, European Pharmacopoeia (EP) Reference Standard