Conversion of the beckman liquid phase sequencer to a gas-liquid phase sequencer.

Analytical biochemistry (1988-11-15)
A S Bhown, J L Wayland, J D Lynn, J C Bennett

As an effective aid to extend the microsequencing capabilities the Beckman protein/peptide sequenator Series 890C has been successfully converted to a gas-liquid system, in which coupling buffer 25% trimethylamine was employed as a gas, and heptafluorobutyric acid as a liquid. The system has been found to be efficient for microsequencing (less than 100 pmol). The details of mechanical, plumbing, and other minor changes are described in this paper along with the results of sequencing proteins and peptides, directly and from blots.

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Heptafluorobutyric acid, ≥99.0% (GC)
Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)