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Determination of chromium in cerebrospinal fluid using electrothermal atomisation atomic absorption spectrometry.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) (2003-01-18)
María Victorina Aguilar, Carmen José Mateos, María Carmen Martínez Para
ABSTRACT

A rapid method to determine the chromium content of cerebrospinal fluid (CSF) samples using electrothermal atomization atomic absorption spectrometry (ETA-AAS) with deuterium-arc background correction is described. The chromium concentration in CSF was evaluated by the standard addition method. Sample dilution (1 + 1) with 0.25% (m/v) Triton X-100 and 4.5% (v/v) HNO3 gave the best combination of sensitivity, reproducibility, and low blank reading compared with dilution using other solvents. Within-batch reproducibility was 3.2% for 20 CSF samples, between-batch reproducibility was 4.7%. CSF samples from 43 healthy volunteers collected in a manner designed to avoid contamination yielded chromium concentrations of 14.6 +/- 6.3 ng mL(-1).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chromium, powder, ≥99% trace metals basis, <45 μm
Sigma-Aldrich
Chromium, powder, 99.5%, −100 mesh
Sigma-Aldrich
Chromium, chips, thickness ~2 mm, 99.5%
Sigma-Aldrich
Chromium, chips, 99.995% trace metals basis
Sigma-Aldrich
Chromium, sputtering target, diam. × thickness 3.00 in. × 0.125 in., 99.95% trace metals basis