Markers of dopamine metabolism in Parkinson's disease. The Parkinson Study Group.

Neurology (1992-11-01)
P A LeWitt, M P Galloway, W Matson, P Milbury, M McDermott, D K Srivastava, D Oakes
RESUMO

We used two analytic methods (a multichannel coulometric electrode array with high-performance liquid chromatography, and gas chromatography-mass spectrophotometry) to measure CSF dopamine (DA) and its metabolites in mildly affected, unmedicated subjects with Parkinson's disease (PD). The mean (+/- SD) concentration of homovanillic acid (HVA), the most abundant product of DA turnover, was 164.57 +/- 95.05 nM. As sequential aliquots of CSF were collected from the first to 23rd ml, CSF HVA concentration almost doubled. After HVA, 3-O-methyldopa (3-O-MD) was the next most abundant compound. The summed concentrations of 3-O-MD, 3,4-dihydroxyphenylacetic acid, 3-methoxytyramine, DA, DA-3-sulfate, homovanillol, and levodopa (LD) amounted to 12.6% of HVA. Concentrations of the DA metabolites did not correlate to a variety of indices of PD severity. The presence of LD and 3-O-MD may be indicators of DA synthesis and possibly could reflect compensatory processes among surviving dopaminergic neurons of the PD brain.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
Taurine, ≥99%
Sigma-Aldrich
Zinc, dust, <10 μm, ≥98%
Sigma-Aldrich
Magnesium, turnings, reagent grade, 98%
Sigma-Aldrich
Zinc, granular, 20-30 mesh, ACS reagent, ≥99.8%
Sigma-Aldrich
Zinc, purum, powder
Sigma-Aldrich
Zinc, powder, <150 μm, 99.995% trace metals basis
Sigma-Aldrich
S-(5′-Adenosyl)-L-homocysteine, crystalline
Sigma-Aldrich
Raney®-Nickel, W.R. Grace and Co. Raney® 2800, slurry, in H2O, active catalyst
Sigma-Aldrich
Calcium, granular, 99%
Sigma-Aldrich
Copper, powder, <425 μm, 99.5% trace metals basis
Sigma-Aldrich
L-Cystine, ≥98% (TLC), crystalline
Sigma-Aldrich
Silicon, powder, −325 mesh, 99% trace metals basis
Sigma-Aldrich
L-Cystine, from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
Manganese, powder, ≥99.9% trace metals basis
Sigma-Aldrich
Taurine, suitable for cell culture, meets USP testing specifications
Sigma-Aldrich
Copper, foil, thickness 0.25 mm, 99.98% trace metals basis
Sigma-Aldrich
DL-Homocysteine, ≥95% (titration)
Sigma-Aldrich
L-Cystine, ≥99.7% (TLC)
Sigma-Aldrich
Chromium, chips, 99.995% trace metals basis
Sigma-Aldrich
Copper, powder, 99.999% trace metals basis
Sigma-Aldrich
Magnesium, ribbon, ≥99.5% Mg basis
Sigma-Aldrich
Magnesium, chips, 6-35 mesh, 99.98% trace metals basis
Sigma-Aldrich
Zinc, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.9%, granular
Sigma-Aldrich
Copper, wire, diam. 1.0 mm, ≥99.9%
Sigma-Aldrich
Tin, ≥99%, powder
Sigma-Aldrich
Zinc, nanopowder, 40-60 nm avg. part. size, ≥99% trace metals basis
Sigma-Aldrich
Nickel, powder, <50 μm, 99.7% trace metals basis
Sigma-Aldrich
Titanium, foil, thickness 0.127 mm, 99.7% trace metals basis
Sigma-Aldrich
(+)-α-Tocopherol, from vegetable oil, Type V, ~1000 IU/g
Sigma-Aldrich
Zinc, foil, thickness 0.25 mm, 99.9% trace metals basis