• Início
  • Resultados da busca
  • A facile light-emitting-diode induced fluorescence detector coupled to an integrated microfluidic device for microchip electrophoresis.

A facile light-emitting-diode induced fluorescence detector coupled to an integrated microfluidic device for microchip electrophoresis.

Talanta (2011-05-03)
Fan Yang, Xin-chun Li, Wen Zhang, Jian-bin Pan, Zuan-guang Chen
RESUMO

In this paper, a compact and inexpensive light emitting diode induced fluorescence (LED-IF) detector with simplified optical configuration was developed and assembled in an integrated microfluidic device for microscale electrophoresis. The facile detector mainly consisted of an LED, a focusing pinhole, an emission filter and a photodiode, and was encapsulated in the upper layer of an aluminum alloy device with two layers. At the bottom layer, integrated circuit (IC) was assembled to manipulate the voltage for sample injection and separation, LED emission and signal amplifying. A high-power LED with fan-shaped heat sink was used as excitation source. The excitation light was focused by a 1.1mm diameter pinhole fabricated in a thin piece of silver foil, and the obtained sensitivity was about 3 times as high as that using electrode plate. Other important parameters including LED driven current, fluorescence collection angle and detection distance have also been investigated. Under optimal conditions, considerable high-response of 0.09 fmol and 0.18 fmol mass detection limits at 0.37 nL injection volume for sodium fluorescein (SF) and FITC was achieved, respectively. This device has been successfully employed to separate penicillamine (PA) enantiomers. Due to such significant features as low-cost, integration, miniaturization, and ease of commercialization, the presented microfluidic device may hold great promise for clinical diagnostics and bioanalytical applications.

MATERIAIS
Número do produto
Marca
Descrição do produto

Silver, foil, light tested, 50x50mm, thickness 0.05mm, annealed, 99.95+%
Silver, foil, 100x100mm, thickness 0.5mm, as rolled, 99.99+%
Silver, foil, 10mm disks, thickness 1.0mm, as rolled, 99.95+%
Silver, foil, 25x25mm, thickness 1.0mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.005mm, 99.97%
Silver, foil, 15mm disks, thickness 0.006mm, 99.97%
Silver, foil, 15mm disks, thickness 0.007mm, 99.97%
Silver, foil, 15mm disks, thickness 0.008mm, 99.95+%
Silver, foil, 15mm disks, thickness 0.008mm, 99.97%
Silver, foil, 15mm disks, thickness 0.009mm, 99.97%
Silver, foil, 15mm disks, thickness 0.0125mm, 99.95+%
Silver, foil, 15mm disks, thickness 0.015mm, 99.97%
Silver, foil, 15mm disks, thickness 0.01mm, 99.95+%
Silver, foil, 15mm disks, thickness 0.025mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.025mm, as rolled, 99.99+%
Silver, foil, 15mm disks, thickness 0.02mm, 99.97%
Silver, foil, 15mm disks, thickness 0.035mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.05mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.075mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.125mm, annealed, 99.95+%
Silver, foil, 15mm disks, thickness 0.125mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.125mm, as rolled, 99.99+%
Silver, foil, 15mm disks, thickness 0.15mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.1mm, annealed, 99.95+%
Silver, foil, 15mm disks, thickness 0.1mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.20mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.25mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.25mm, as rolled, 99.99+%
Silver, foil, 15mm disks, thickness 0.5mm, as rolled, 99.95+%
Silver, foil, 15mm disks, thickness 0.5mm, as rolled, 99.99+%

Redes sociais

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Pesquisa. Desenvolvimento. Produção.

Somos um fornecedor global líder para o setor de ciências biológicas, com soluções e serviços para pesquisa, desenvolvimento e produção de biotecnologia, além de produção e desenvolvimento de terapias farmacológicas com medicamentos.

© 2021 Merck KGaA, Darmstadt, Alemanha e/ou suas filiais. Todos os direitos reservados.

A reprodução não autorizada de quaisquer materiais deste site é estritamente proibida.