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Optimizing fluorescent protein choice for transgenic embryonic medaka models.

Environmental toxicology and chemistry (2013-07-09)
Nicolas Loire, Emilie Barbeau, Gregory F Lemkine, Marc A Léonard, Andrew J Tindall
ABSTRACT

Early-life-stage transgenic medaka are recognized as a pertinent model by the Organisation for Economic Co-operation and Development and are noncompliant with the European definition of a laboratory animal. However, autofluorescence confounds readout of fluorescent biomarkers. The authors determined the fluorescence emission spectrum of different embryonic stages of medaka submitted to a range of excitation wavelengths. This allows selection of high signal-to-noise ratio fluorescent proteins and combining multiple biomarkers within a single embryo.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methylene Blue solution, for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution
Sigma-Aldrich
Methylene Blue solution, suitable for microbiology
Sigma-Aldrich
Methylene Blue solution, for microscopy