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  • Neolignan Licarin A presents effect against Leishmania (Leishmania) major associated with immunomodulation in vitro.

Neolignan Licarin A presents effect against Leishmania (Leishmania) major associated with immunomodulation in vitro.

Experimental parasitology (2013-07-31)
Patrícia L N Néris, John P A Caldas, Yara K S Rodrigues, Francianne M Amorim, Jacqueline A Leite, Sandra Rodrigues-Mascarenhas, José M Barbosa-Filho, Luis C Rodrigues, Márcia R Oliveira
ABSTRACT

Leishmaniasis' treatment is based mostly on pentavalent antimonials or amphotericin B long-term administration, expensive drugs associated with severe side effects. Considering these aforementioned, the search for alternative effective and safe leishmaniasis treatments is a necessity. This work evaluated a neolignan, licarin A anti-leishmanial activity chemically synthesized by our study group. It was observed that licarin A effectively inhibited Leishmania (Leishmania) major promastigotes (IC₅₀ of 9.59 ± 0.94 μg/mL) growth, by inducing in these parasites genomic DNA fragmentation in a typical death pattern by apoptosis. Additionally, the neolignan proved to be even more active against intracellular amastigotes of the parasite (EC₅₀ of 4.71 ± 0.29 μg/mL), and significantly more effective than meglumine antimoniate (EC₅₀ of 216.2 ± 76.7 μg/mL) used as reference drug. The antiamastigote activity is associated with an immunomodulatory activity, since treatment with licarin A of the infected macrophages induced a decrease in the interleukin (IL)-6 and IL-10 production. This study demonstrates for the first time the antileishmanial activity of licarin A and suggests that the compound may be a promising in the development of a new leishmanicidal agent.

MATERIALS
Product Number
Brand
Product Description

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