Merck

Functional involvement of RFVT3/SLC52A3 in intestinal riboflavin absorption.

American journal of physiology. Gastrointestinal and liver physiology (2013-11-23)
Hiroki Yoshimatsu, Atsushi Yonezawa, Yoshiaki Yao, Kumiko Sugano, Shunsaku Nakagawa, Tomohiro Omura, Kazuo Matsubara
RESUMO

Riboflavin, also known as vitamin B2, is transported across the biological membrane into various organs by transport systems. Riboflavin transporter RFVT3 is expressed in the small intestine and has been suggested to localize in the apical membranes of the intestinal epithelial cells. In this study, we investigated the functional involvement of RFVT3 in riboflavin absorption using intestinal epithelial T84 cells and mouse small intestine. T84 cells expressed RFVT3 and conserved unidirectional riboflavin transport corresponding to intestinal absorption. Apical [(3)H]riboflavin uptake was pH-dependent in T84 cells. This uptake was not affected by Na(+) depletion at apical pH 6.0, although it was significantly decreased at apical pH 7.4. The [(3)H]riboflavin uptake from the apical side of T84 cells was prominently inhibited by the RFVT3 selective inhibitor methylene blue and significantly decreased by transfection of RFVT3-small-interfering RNA. In the gastrointestinal tract, RFVT3 was expressed in the jejunum and ileum. Mouse jejunal and ileal permeabilities of [(3)H]riboflavin were measured by the in situ closed-loop method and were significantly reduced by methylene blue. These results strongly suggest that RFVT3 would functionally be involved in riboflavin absorption in the apical membranes of intestinal epithelial cells.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
(−)-Riboflavin, from Eremothecium ashbyii, ≥98%
Sigma-Aldrich
(−)-Riboflavin, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%
Sigma-Aldrich
(−)-Riboflavin, meets USP testing specifications
Supelco
Riboflavin, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Riboflavin (B2), analytical standard
Sigma-Aldrich
Methylene Blue solution, for microscopy
Sigma-Aldrich
Methylene Blue solution, suitable for microbiology
Sigma-Aldrich
Methylene Blue solution, for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution