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  • Role of insulin-like growth factor II receptor in transdifferentiation of free silica-induced primary rat lung fibroblasts.

Role of insulin-like growth factor II receptor in transdifferentiation of free silica-induced primary rat lung fibroblasts.

Biomedical and environmental sciences : BES (2014-01-08)
Chang Fu Hao, Xiao Fang Li, Wu Yao
RESUMO

To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts. Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of α-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin. The expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used. The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.

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