Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)
Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya
RESUMO

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
Raney®-Nickel, W.R. Grace and Co. Raney®2800, slurry, in H2O, active catalyst
Sigma-Aldrich
Nickel, powder, <50 μm, 99.7% trace metals basis
Sigma-Aldrich
Nickel, nanopowder, <100 nm avg. part. size, ≥99% trace metals basis
Sigma-Aldrich
Nickel, powder, <150 μm, 99.99% trace metals basis
Sigma-Aldrich
Nickel, foil, thickness 0.125 mm, ≥99.9%
Nickel, foam, 150x150mm, thickness 1.6mm, bulk density 0.45g/cm3, porosity 95%, 95%
Sigma-Aldrich
Nickel, powder, <1 μm, 99.8% trace metals basis
Sigma-Aldrich
Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis
Sigma-Aldrich
Raney®-Nickel, W.R. Grace and Co. Raney®2400, slurry, in H2O, active catalyst
Sigma-Aldrich
Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis
Sigma-Aldrich
Nickel, wire, diam. 0.25 mm, ≥99.9%
Sigma-Aldrich
Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis
Sigma-Aldrich
Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis
Nickel, tube, 500mm, outside diameter 1.8mm, inside diameter 1.6mm, wall thickness 0.1mm, hard, 99.5%
Nickel, wire reel, 20m, diameter 0.025mm, as drawn, 99.98%
Nickel, wire reel, 1m, diameter 1.0mm, as drawn, 99%
Nickel, mesh, 100x250mm, nominal aperture 0.73mm, wire diameter 0.25mm, 26x26 wires/inch, open area 55%, plain weave mesh, 99%
Nickel, sphere, 100pcs, diameter 1.0mm, precision sphere grade 200, 99%
Nickel, foil, 50x50mm, thickness 0.30mm, as rolled, 99.98%
Nickel, wire reel, 5m, diameter 1.0mm, hard, 99.98%
Nickel, wire reel, 1m, diameter 0.75mm, as drawn, 99%
Nickel, rod, 100mm, diameter 12.7mm, 99.98%
Nickel, sphere, 200pcs, diameter 3.0mm, precision sphere grade 200, 99%
Nickel, foil, light tested, 100x100mm, thickness 0.015mm, 99.9%
Nickel, foil, 10mm disks, thickness 0.15mm, annealed, 99%
Nickel, foil, 4mm disks, thickness 0.075mm, annealed, 99%
Nickel, foil, 4mm disks, thickness 0.075mm, as rolled, 99.98%
Nickel, foil, 4mm disks, thickness 0.125mm, annealed, 99%
Nickel, foil, 4mm disks, thickness 0.125mm, annealed, 99.98%

Redes sociais

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Pesquisa. Desenvolvimento. Produção.

Somos um fornecedor global líder para o setor de ciências biológicas, com soluções e serviços para pesquisa, desenvolvimento e produção de biotecnologia, além de produção e desenvolvimento de terapias farmacológicas com medicamentos.

© 2021 Merck KGaA, Darmstadt, Alemanha e/ou suas filiais. Todos os direitos reservados.

A reprodução não autorizada de quaisquer materiais deste site é estritamente proibida.