Merck

X-ray crystal structure of voltage-gated proton channel.

Nature structural & molecular biology (2014-03-04)
Kohei Takeshita, Souhei Sakata, Eiki Yamashita, Yuichiro Fujiwara, Akira Kawanabe, Tatsuki Kurokawa, Yoshifumi Okochi, Makoto Matsuda, Hirotaka Narita, Yasushi Okamura, Atsushi Nakagawa
RESUMO

The voltage-gated proton channel Hv1 (or VSOP) has a voltage-sensor domain (VSD) with dual roles of voltage sensing and proton permeation. Its gating is sensitive to pH and Zn(2+). Here we present a crystal structure of mouse Hv1 in the resting state at 3.45-Å resolution. The structure showed a 'closed umbrella' shape with a long helix consisting of the cytoplasmic coiled coil and the voltage-sensing helix, S4, and featured a wide inner-accessible vestibule. Two out of three arginines in S4 were located below the phenylalanine constituting the gating charge-transfer center. The extracellular region of each protomer coordinated a Zn(2+), thus suggesting that Zn(2+) stabilizes the resting state of Hv1 by competing for acidic residues that otherwise form salt bridges with voltage-sensing positive charges on S4. These findings provide a platform for understanding the general principles of voltage sensing and proton permeation.

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Zinc, foil, 10mm disks, thickness 1.0mm, as rolled, 99.99+%
Zinc, foil, 15mm disks, thickness 0.5mm, as rolled, 99.99+%
Zinc, foil, 10mm disks, thickness 0.025mm, as rolled, 99.99+%
Zinc, foil, 15mm disks, thickness 0.075mm, as rolled, 99.95+%
Zinc, foil, 10mm disks, thickness 0.175mm, as rolled, 99.95+%
Zinc, foil, 1m coil, thickness 0.5mm, as rolled, 99.95+%
Zinc, foil, 10mm disks, thickness 0.005mm, 99.9%
Zinc, foil, 0.3m coil, thickness 0.25mm, as rolled, 99.95+%
Zinc, foil, 15mm disks, thickness 1.0mm, as rolled, 99.95+%
Zinc, foil, 15mm disks, thickness 0.15mm, as rolled, 99.95+%
Zinc, foil, 0.3m coil, thickness 0.075mm, as rolled, 99.95+%
Zinc, foil, 10mm disks, thickness 0.5mm, as rolled, 99.99+%
Zinc, foil, 50x50mm, thickness 0.5mm, as rolled, 99.99+%
Zinc, foil, 15mm disks, thickness 0.05mm, as rolled, 99.95+%
Zinc, foil, 10mm disks, thickness 0.125mm, as rolled, 99.99+%
Zinc, foil, 0.5m coil, thickness 0.1mm, as rolled, 99.95+%
Zinc, foil, 10mm disks, thickness 0.25mm, as rolled, 99.95+%
Zinc, foil, 0.5m coil, thickness 1.0mm, as rolled, 99.95+%
Zinc, foil, 50x50mm, thickness 0.35mm, as rolled, 99.95+%
Zinc, foil, 15mm disks, thickness 0.02mm, 99.9%
Zinc, foil, 150x150mm, thickness 1.0mm, as rolled, 99.99+%
Zinc, foil, 0.5m coil, thickness 1.5mm, as rolled, 99.95+%
Zinc, foil, 15mm disks, thickness 0.35mm, as rolled, 99.95+%
Zinc, foil, 10mm disks, thickness 0.01mm, 99.9%