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Bioconjugation of poly(acrylic acid)-capped BaYF5:Yb3+/Er3+ up-conversion nanoparticles to bovine serum albumin: synthesis and photoluminescent properties.

Journal of nanoscience and nanotechnology (2014-04-17)
Wanyue Shao, Zhengang Sun, Ruinian Hua, Wei Zhang, Jun Zhao, Liyan Na
ABSTRACT

Water-soluble BaYF5:Yb3+/Er3+ nanoparticles with the surface functionalized by a layer of poly(acrylic acid) (PAA) were synthesized via a facile one-step PAA-assisted hydrothermal method. Bovine serum albumin (BSA) protein was conjugated with BaYF5:Yb3+/Er3+ upconversion nanoparticles via free carboxylic acid groups on the surface of nanoparticles. The final products were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), infrared (IR) spectrophotometry, ultraviolet (UV) spectrophotometry and photoluminescence spectroscopy (PL). The XRD results showed that PAA-capped BaYF5:Yb3+/Er3+ upconversion nanoparticles could be obtained via a PAA assisted hydrothermal process with the pH value of 8 at 200 degrees C for 24 h. The TEM results showed that the morphology of BaYF5:Yb3+/Er3+ nanoparticles was spherical particles with an average diameter of about 4 nm. The IR and UV spectra showed that BSA has been conjugated with BaYF5:Yb3+/Er3+ up-conversion nanoparticles. The luminescence properties of BaYF5:Yb3+/Er3+ up-conversion nanoparticles were also studied. The luminescence properties of the products suggest that BaYF5:Yb3+/Er3+ upconversion nanoparticles have promising applications for labels in biological assays.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
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Ytterbium, ingot, 99.9% trace rare earth metals basis
Ytterbium, foil, 25x25mm, thickness 0.125mm, as rolled, 99%
Ytterbium, foil, not light tested, 25x25mm, thickness 0.005mm, as rolled, 99%
Ytterbium, lump, 50g, max. size 25mm, 99.9%
Ytterbium, foil, 25x25mm, thickness 0.25mm, as rolled, 99%
Ytterbium, rod, 100mm, diameter 10.0mm, cast, 99%
Ytterbium, rod, 50mm, diameter 10.0mm, cast, 99%
Ytterbium, foil, 50x50mm, thickness 0.25mm, as rolled, 99%
Ytterbium, rod, 50mm, diameter 2.0mm, cast, 99%
Ytterbium, rod, 100mm, diameter 6.35mm, cast, 99%
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Ytterbium, foil, not light tested, 25x25mm, thickness 0.025mm, as rolled, 99%
Ytterbium, foil, not light tested, 50x50mm, thickness 0.025mm, as rolled, 99%
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Ytterbium, lump, 100g, max. size 25mm, 99.9%
Ytterbium, lump, 25g, max. size 25mm, 99.9%
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Erbium, rod, 50mm, diameter 2.0mm, cast, 99%
Erbium, foil, not light tested, 25x25mm, thickness 0.005mm, as rolled, 99%
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Erbium, wire reel, 100mm, diameter 1.0mm, hard, 99.9%
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