• Início
  • Resultados da busca
  • Quantitative biokinetics and systemic translocation of various gold nanostructures are highly dependent on their size and shape.

Quantitative biokinetics and systemic translocation of various gold nanostructures are highly dependent on their size and shape.

Journal of nanoscience and nanotechnology (2014-04-18)
Jiajing Zhang, Xin Nie, Yinglu Ji, Ying Liu, Xiaochun Wu, Chunying Chen, Xiaohong Fang
RESUMO

Gold nanostructures with promising applications in biomedical field have attracted great attention. However, some fundamental questions other than the development of novel applications should be elucidated before they can actually serve as biomedicines in the clinic. Bio-safety is one of the most important issues. Since numerous modifications (e.g., surface coating and composites) have been designed on gold nanoparticles (GNPs) to extend their application, there would be hundreds of GNPs synthesized in the lab although the prototypes of GNPs (i.e., cluster, shell, rod, sphere, cage, and star) are rather limited. Thus, in the present work we aim to conduct our experiments only on the most basic types of GNPs--including gold nanocluster (GNC), gold nanorod (GNR) and gold nanosphere (GNS), to investigate their biodistribution and toxicities in vivo, in the hope of revealing some basic rules which could be further extended to other complicated situations. Bovine serum albumin (BSA) was coated at the surface to increase their plasma stability and of the same BSA coating would help to compare the fate and behaviors of various GNPs in vivo. After intravenous administration of different GNPs with an equal content of gold element at 0.5 mg/kg in mice, samples were harvested at a series of time points. Biodistribution was compared among different GNPs and the process of accumulation-retention-clearance of each kind of GNP was also observed through quantification analysis by inductively coupled plasma-mass spectrometry (ICP-MS). The results showed, with the same BSA coating at the surface and similar negative charge, size rather than shape was dominating the in vivo fate of GNPs. Even between GNC and hydrolyzed GNC with their size at 7.1 and 3.2 nm, huge difference in the kidney accumulation was observed. Totally, GNR and GNS in relative large size preferred to accumulate in liver and spleen whereas GNC in relative small size tended to accumulate in liver and kidney. GNPs resided in liver were hardly cleared out of body till 28 d whereas their accumulation in kidney was almost entirely eliminated with prolonged time, although not as rapid as reported in previous work. In vivo toxicities evaluated by pathology observation and blood biochemical analysis also revealed slight liver and kidney damage, basically associated with the biodistribution pattern of GNPs.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
Gold, nanopowder, <100 nm particle size, 99.9% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 0.25 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Gold, foil, thickness 0.127 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, powder, <10 μm, ≥99.9% trace metals basis
Sigma-Aldrich
Gold, powder, <45 μm, 99.99% trace metals basis
Sigma-Aldrich
Gold coated microscope slide, layer thickness 100 Å, 99.999% (Au)
Sigma-Aldrich
Gold, beads, 1-6 mm, 99.999% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 1.0 mm, 99.997% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 0.5 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, foil, thickness 0.1 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 0.1 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, foil, thickness 0.025 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold coated microscope slide, layer thickness 1000 Å, 99.999% (Au)
Sigma-Aldrich
Gold, evaporation slug, diam. × L 0.3 cm × 0.6 cm, 99.99% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 1.0 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold coated glass cover slip, 99.999% (Au), layer thickness 100 Å, L × W × thickness 22 mm × 22 mm × 130-170 μm, square
Sigma-Aldrich
Gold, evaporation slug, diam. × L 0.6 cm × 1.2 cm, 99.99% trace metals basis
Sigma-Aldrich
Gold, foil, thickness 0.05 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, foil, thickness 0.25 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 0.127 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 2.0 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, powder, <850 μm, ≥99.99% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 0.25 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 0.5 mm, 99.999% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 1.5 mm, 99.999% trace metals basis
Gold, insulated wire, 0.5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
Gold, tube, 100mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%
Gold, insulated wire, 0.1m, conductor diameter 0.10mm, insulation thickness 0.013mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Gold, insulated wire, 0.1m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Gold, insulated wire, 0.2m, conductor diameter 0.025mm, insulation thickness 0.002mm, PTFE (polytetrafluoroethylene) insulation, 99.99%