• Início
  • Resultados da busca
  • Aptamer-fluorescent silica nanoparticles bioconjugates based dual-color flow cytometry for specific detection of Staphylococcus aureus.

Aptamer-fluorescent silica nanoparticles bioconjugates based dual-color flow cytometry for specific detection of Staphylococcus aureus.

Journal of biomedical nanotechnology (2014-05-09)
Xiaoxiao He, Yuhong Li, Dinggen He, Kemin Wang, Jingfang Shangguan, Hui Shi
RESUMO

This paper describes a sensitive and specific determination strategy for Staphylococcus aureus (S. aureus) detection using aptamer recognition and fluorescent silica nanoparticles (FSiNPs) label based dual-color flow cytometry assay (Aptamer/FSiNPs-DCFCM). In the protocol, an aptamer, having high affinity to S. aureus, was first covalently immobilized onto chloropropyl functionalized FSiNPs through a click chemistry approach to generate aptamer-nanoparticles bioconjugates (Aptamer/FSiNPs). Next, S. aureus was incubated with Aptamer/FSiNPs, and then stained with SYBR Green I (a special staining material for the duplex DNA). Upon target binding and nucleic acid staining with SYBR Green I, the S. aureus was determined using two-color flow cytometry. The method took advantage of the specificity of aptamer, signal amplification of FSiNPs label and decreased false positives of two-color flow cytometry assay. It was demonstrated that these Aptamer/FSiNPs could efficiently recognize and fluorescently label target S. aureus. Through multiparameter determination with flow cytometry, this assay allowed for detection of as low as 1.5 x 10(2) and 7.6 x 10(2) cells mL(-1) S. aureus in buffer and spiked milk, respectively, with higher sensitivity than the Aptamer/FITC based flow cytometry.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
Silicon dioxide, nanopowder, 10-20 nm particle size (BET), 99.5% trace metals basis
Sigma-Aldrich
LUDOX® HS-40 colloidal silica, 40 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® AS-40 colloidal silica, 40 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® TM-50 colloidal silica, 50 wt. % suspension in H2O
Sigma-Aldrich
Silica, nanoparticles, mesoporous, 200 nm particle size, pore size 4 nm
Sigma-Aldrich
Silica, mesostructured, MCM-41 type (hexagonal)
Sigma-Aldrich
Fluorescein 5(6)-isothiocyanate, BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Sigma-Aldrich
Silicon dioxide, nanopowder (spherical, porous), 5-20 nm particle size (TEM), 99.5% trace metals basis
Sigma-Aldrich
LUDOX® AM colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
Silica, nanopowder, spec. surface area 175-225 m2/g (BET), 99.8% trace metals basis
Sigma-Aldrich
LUDOX® SM colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® HS-30 colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® TM-40 colloidal silica, 40 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® TMA colloidal silica, 34 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® AS-30 colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
Sigma-Aldrich
LUDOX® CL colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® LS colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
Silica
Sigma-Aldrich
Silica, mesostructured, MSU-F (cellular foam)
Sigma-Aldrich
LUDOX® CL-X colloidal silica, 45 wt. % suspension in H2O