Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil.

Food chemistry (2014-02-13)
Abdul Azis Ariffin, H M Ghazali, Parviz Kavousi
RESUMO

For the first time 5-hydroxymethyl-2-furaldehyde (HMF) was separated from crude palm oil (CPO), and its authenticity was determined using an RP-HPLC method. Separation was accomplished with isocratic elution of a mobile phase comprising water and methanol (92:8 v/v) on a Purospher Star RP-18e column (250mm×4.6mm, 5.0μm). The flow rate was adjusted to 1ml/min and detection was performed at 284nm. The method was validated, and results obtained exhibit a good recovery (95.58% to 98.39%). Assessment of precision showed that the relative standard deviations (RSD%) of retention times and peak areas of spiked samples were less than 0.59% and 2.66%, respectively. Further, the limit of detection (LOD) and LOQ were 0.02, 0.05mg/kg, respectively, and the response was linear across the applied ranges. The crude palm oil samples analysed exhibited HMF content less than 2.27mg/kg.

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Sigma-Aldrich
Furfural, 99%
Sigma-Aldrich
5-(Hydroxymethyl)furfural, ≥99%, FG
Sigma-Aldrich
5-Hydroxymethyl-2-furaldehyde, 99%
Supelco
5-(Hydroxymethyl)furfural, analytical standard
Supelco
Furfural, analytical standard
Sigma-Aldrich
Furfural, ≥98%, FCC, FG
Sigma-Aldrich
Furfural, natural, ≥98%, FCC, FG
Supelco
Palm oil, analytical standard
Sigma-Aldrich
Furfural, ACS reagent, 99%

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