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  • Dietary grape powder increases IL-1β and IL-6 production by lipopolysaccharide-activated monocytes and reduces plasma concentrations of large LDL and large LDL-cholesterol particles in obese humans.

Dietary grape powder increases IL-1β and IL-6 production by lipopolysaccharide-activated monocytes and reduces plasma concentrations of large LDL and large LDL-cholesterol particles in obese humans.

The British journal of nutrition (2014-05-17)
Susan J Zunino, Jan M Peerson, Tammy L Freytag, Andrew P Breksa, Ellen L Bonnel, Leslie R Woodhouse, David H Storms
RESUMO

Obese individuals are at an increased risk of developing CVD, hypertension, type 2 diabetes, and bacterial and viral infections when compared with the normal-weight population. In a 9-week randomised, double-blind, cross-over study, twenty-four obese subjects aged between 20 and 60 years and with a BMI between 30 and 45 kg/m2 were fed grape or placebo powder for 3-week intervals to determine the effects of dietary grapes on blood lipid profiles, plasma inflammatory marker concentrations and immune cell function. Blood samples were collected on days 1 and 8 for obtaining baseline information and at weeks 3, 4, 8 and 9. Comprehensive chemistry panels, lipid profile analyses by NMR, measurement of plasma inflammatory marker concentrations, and analyses of cytokine production by activated T lymphocytes and monocytes were performed for each blood draw. Dietary grape powder reduced the plasma concentrations of large LDL-cholesterol and large LDL particles compared with the placebo powder (P< 0·05). The concentrations of interferon-γ, TNF-α, IL-4 and IL-10 were measured in supernatants from peripheral blood mononuclear cells (PBMC) activated with anti-CD3/CD28 antibodies and those of TNF-α, IL-1β, IL-6 and IL-8 were measured in supernatants from PBMC activated with lipopolysaccharide (LPS). No difference in the production of T-cell cytokines was observed between the two intervention groups. The production of IL-1β and IL-6 was increased in supernatants from LPS-activated PBMC in the grape powder group compared with the placebo powder group (P< 0·05). These data suggest that dietary grapes may decrease atherogenic lipid fractions in obese individuals and increase the sensitivity of monocytes in a population at a greater risk of developing infections.

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Sigma-Aldrich
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Zinc, foil, 25x25mm, thickness 1.0mm, as rolled, 99.95+%
Zinc, foil, 150x150mm, thickness 0.20mm, as rolled, 99.95+%
Zinc, foil, 50x50mm, thickness 0.15mm, as rolled, 99.95+%
Zinc, foil, 25x25mm, thickness 1.0mm, as rolled, 99.99+%
Zinc, foil, 150x150mm, thickness 0.25mm, as rolled, 99.95+%
Zinc, foil, 0.5m coil, thickness 0.05mm, as rolled, 99.95+%
Zinc, foil, 150x150mm, thickness 0.35mm, as rolled, 99.95+%
Zinc, foil, 25x25mm, thickness 1.5mm, as rolled, 99.95+%
Zinc, foil, 25x25mm, thickness 0.25mm, as rolled, 99.95+%
Zinc, foil, 50x50mm, thickness 0.1mm, as rolled, 99.95+%
Zinc, foil, 25x25mm, thickness 2.0mm, as rolled, 99.95+%
Zinc, foil, 25x25mm, thickness 6mm, as rolled, 99.99+%
Zinc, foil, 10mm disks, thickness 0.25mm, as rolled, 99.99+%
Zinc, foil, 10mm disks, thickness 0.02mm, 99.9%
Zinc, foil, 1m coil, thickness 0.025mm, as rolled, 99.95+%
Zinc, foil, 500x1000mm, thickness 1.0mm, as rolled, 99.7%
Zinc, foil, 50x50mm, thickness 0.20mm, as rolled, 99.95+%
Zinc, foil, 15mm disks, thickness 0.1mm, as rolled, 99.95+%
Zinc, foil, 300x300mm, thickness 0.125mm, as rolled, 99.95+%
Zinc, foil, 300x300mm, thickness 0.35mm, as rolled, 99.95+%
Zinc, foil, 0.3m coil, thickness 0.05mm, as rolled, 99.95+%
Zinc, foil, 0.5m coil, thickness 0.025mm, as rolled, 99.99+%
Zinc, foil, 10mm disks, thickness 0.125mm, as rolled, 99.95+%
Zinc, foil, 15mm disks, thickness 0.25mm, as rolled, 99.95+%