Binding between lead ions and the high-abundance serum proteins.

Chemosphere (2014-07-23)
Ming Guo, Ling He, P J Strong, Hailong Wang
RESUMO

The interaction between three of the most abundant bovine serum proteins (serum albumin, transferrin and IgG) with Pb(2+) was investigated using electrochemistry. The data was used to construct a new theoretical model of Pb(2+) binding to the high-abundance serum proteins under non-ideal conditions. The binding constants (β) of Pb(2+) to the individual proteins and a mixture of proteins were measured according to a new theoretical equation (non-ideal state) as well as the McGhee-Von Hippel equation (ideal state). Differences between the models suggested that the β values obtained using the non-ideal state model was more realistic. Protein-protein interactions and micro-environmental influences affected binding between Pb(2+) and the high-abundance serum proteins. We included a micro-environmental influence factor for the model (Fm), which accurately quantified the effect of micro-environment of the proteome of Pb(2+) binding with the serum proteins. This research provides a useful reference of theoretical and experimental work regarding heavy-metal binding interactions with serum proteins.

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Sigma-Aldrich
Lead, powder, −100 mesh, 99.95% trace metals basis
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Lead, shot, 1-3 mm, 99.995% trace metals basis
Lead, foil, 150x150mm, thickness 0.5mm, as rolled, 99.95%
Lead, foil, 25x25mm, thickness 1.0mm, as rolled, 99.95%
Lead, rod, 200mm, diameter 6.35mm, 99.95%
Lead, foil, 300x300mm, thickness 0.25mm, as rolled, 99.95%
Lead, foil, 150x150mm, thickness 0.1mm, as rolled, 99.95%
Lead, foil, 50x50mm, thickness 0.5mm, as rolled, 99.95%
Lead, wire reel, 1m, diameter 0.75mm, as drawn, 99.99+%
Lead, foil, 100x100mm, thickness 0.5mm, as rolled, 99.95%
Lead, foil, 50x50mm, thickness 0.125mm, as rolled, 99.99+%
Lead, foil, 100x100mm, thickness 1.0mm, as rolled, 99.95%
Lead, foil, 100x100mm, thickness 3.0mm, as rolled, 99.95%
Lead, foil, 100x100mm, thickness 3.0mm, as rolled, 99.99+%
Lead, foil, 100x100mm, thickness 6.0mm, as rolled, 99.99+%
Lead, foil, 150x150mm, thickness 0.125mm, as rolled, 99.95%
Lead, foil, 150x150mm, thickness 0.15mm, as rolled, 99.95%
Lead, foil, 150x150mm, thickness 0.25mm, as rolled, 99.95%
Lead, foil, 150x150mm, thickness 0.55mm, as rolled, 99.999%
Lead, foil, 150x150mm, thickness 1.0mm, as rolled, 99.5%
Lead, foil, 150x150mm, thickness 1.0mm, as rolled, 99.95%
Lead, foil, 250x250mm, thickness 3.0mm, as rolled, 99.95%
Lead, foil, 25x25mm, thickness 0.125mm, as rolled, 99.95%
Lead, foil, 25x25mm, thickness 0.125mm, as rolled, 99.99+%
Lead, foil, 25x25mm, thickness 0.15mm, as rolled, 99.95%
Lead, foil, 25x25mm, thickness 0.1mm, as rolled, 99.95%
Lead, foil, 25x25mm, thickness 0.25mm, as rolled, 99.95%