Merck
  • Home
  • Search Results
  • Vacuolar-ATPase isoform a2 regulates macrophages and cytokine profile necessary for normal spermatogenesis in testis.

Vacuolar-ATPase isoform a2 regulates macrophages and cytokine profile necessary for normal spermatogenesis in testis.

Journal of leukocyte biology (2014-03-14)
Mukesh K Jaiswal, Gajendra K Katara, Timothy Mallers, Gerard Chaouat, Alice Gilman-Sachs, Kenneth D Beaman
ABSTRACT

a2V is required for maturation of sperm. The decreased expression of a2V at the feto-maternal interphase causes poor pregnancy outcome. The present study examined the role of a2V in spermatogenesis and inflammatory network in the testis. A single dose of anti-a2V mouse IgG or mouse IgG isotype (3 μg/animal) was injected i.p. into male mice on alternate days for 10 days. Anti-a2V-treated males exhibit severe deficiencies of spermatogenesis, which is indicated by the presence of less numbers of postmeiotic cells. Sperm counts and sperm motility were reduced significantly in anti-a2V-treated males. The release of the cleaved a2NTD was significantly lower in anti-a2V-treated testes. The TMs were identified as M2-like macrophages, and this population and the expression of various cytokines/chemokines (Tgf-β, Il-6, Nos2, Tnf, Lif, Mcp1, Ccl5) were decreased significantly in anti-a2V-treated testis compared with control testis. Moreover, the cleaved a2NTD acts as a key mediator of TMs and significantly up-regulates the secretion of testicular cytokines/chemokines, which are associated with normal spermatogenesis. When these anti-a2V-treated males were used for mating with normal females, the number of implantation sites was decreased significantly in the females mated with anti-a2V-treated males than the females mated with control males. These observations suggest that a2V plays a crucial role in spermatogenesis by regulating testicular immune responses, and its inhibition in males leads to poor pregnancy outcome in females.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Silicon dioxide, purum p.a., acid purified, sand
Sigma-Aldrich
Silica, mesostructured, SBA-15, 99% trace metals basis
Sigma-Aldrich
Silicon dioxide, purum p.a., acid purified
Sigma-Aldrich
Silicon dioxide, ~99%, 0.5-10 μm (approx. 80% between 1-5 μm)
Sigma-Aldrich
Silica, mesostructured, MCM-41 type (hexagonal)
Sigma-Aldrich
Silicon dioxide, nanopowder, 10-20 nm particle size (BET), 99.5% trace metals basis
Sigma-Aldrich
Silicon dioxide, nanopowder (spherical, porous), 5-20 nm particle size (TEM), 99.5% trace metals basis
Sigma-Aldrich
Silicon dioxide, single crystal substrate, optical grade, 99.99% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm
Sigma-Aldrich
Silica, nanoparticles, mesoporous, 200 nm particle size, pore size 4 nm
Sigma-Aldrich
Silica, fumed, powder
Sigma-Aldrich
Silica, fumed, powder, 0.2-0.3 μm avg. part. size (aggregate)
Sigma-Aldrich
LUDOX® CL colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® SM colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
1-Naphthyl phosphate monosodium salt monohydrate, ≥98% (titration), powder
Sigma-Aldrich
Silicon dioxide, granular, ≥99.9%
Sigma-Aldrich
Silicon dioxide, acid washed
Sigma-Aldrich
Silica, mesostructured, MSU-F (cellular foam)
Sigma-Aldrich
Silica, nanopowder, spec. surface area 175-225 m2/g (BET), 99.8% trace metals basis
Sigma-Aldrich
LUDOX® HS-30 colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® TM-40 colloidal silica, 40 wt. % suspension in H2O
Sigma-Aldrich
Silicon dioxide, −325 mesh, 99.5% trace metals basis
Sigma-Aldrich
Silica
Sigma-Aldrich
LUDOX® LS colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
Silicon dioxide, fused (granular), 4-20 mesh, 99.9% trace metals basis
Sigma-Aldrich
Silicon dioxide, fused (pieces), 4 mm, 99.99% trace metals basis
Sigma-Aldrich
Silicon dioxide, washed and calcined, analytical reagent
Sigma-Aldrich
Silicon dioxide, acid washed and calcined, Analytical Reagent
Supelco
Silica, 99.8%
Supelco
Glass spheres
Sigma-Aldrich
β-D-Allose, rare aldohexose sugar