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Bimetallic Pd-Pt supported graphene promoted enzymatic redox cycling for ultrasensitive electrochemical quantification of microRNA from cell lysates.

The Analyst (2014-07-01)
Fang-Fang Cheng, Jing-Jing Zhang, Ting-Ting He, Jian-Jun Shi, E S Abdel-Halim, Jun-Jie Zhu
ABSTRACT

The expression of microRNAs (miRNAs) is related to some cancer diseases. Recently, miRNAs have emerged as new candidate diagnostic and prognostic biomarkers for detecting a wide variety of cancers. Due to low levels, short sequences and high sequence homology among family members, the quantitative miRNA analysis is still a challenge. A novel electrochemical biosensor with triple signal amplification for the ultrasensitive detection of miRNA was developed based on phosphatase, redox-cycling amplification, a bimetallic Pd-Pt supported graphene functionalized screen-printed gold electrode, and two stem-loop structured DNAs as target capturers. The proposed biosensor is highly sensitive due to the enhanced electrochemical signal of Pd-Pt supported graphene and sufficiently selective to discriminate the target miRNA from homologous miRNAs in the presence of loop-stem structure probes with T4 DNA ligase. Therefore, this strategy provided a new and ultrasensitive platform for amplified detection and subsequent analysis of miRNA in biomedical research and clinical diagnosis.

MATERIALS
Product Number
Brand
Product Description

Supelco
Tris(2-carboxyethyl)phosphine hydrochloride solution, 0.5 M, pH 7.0(aqueous solution; pH was adjusted with ammonium hydroxide)
Sigma-Aldrich
Dopamine hydrochloride
Sigma-Aldrich
Tris(2-carboxyethyl)phosphine hydrochloride, powder
Supelco
Dopamine hydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Tris(2-carboxyethyl)phosphine hydrochloride, BioUltra, ≥98% (NMR)
Dopamine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Tris(2-carboxyethyl)phosphine hydrochloride, BioUltra, suitable for electrophoresis, SDS-PAGE tested