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Lactobacillus rossiae, a vitamin B12 producer, represents a metabolically versatile species within the Genus Lactobacillus.

PloS one (2014-09-30)
Maria De Angelis, Francesca Bottacini, Bruno Fosso, Philip Kelleher, Maria Calasso, Raffaella Di Cagno, Marco Ventura, Ernesto Picardi, Douwe van Sinderen, Marco Gobbetti
ABSTRACT

Lactobacillus rossiae is an obligately hetero-fermentative lactic acid bacterium, which can be isolated from a broad range of environments including sourdoughs, vegetables, fermented meat and flour, as well as the gastrointestinal tract of both humans and animals. In order to unravel distinctive genomic features of this particular species and investigate the phylogenetic positioning within the genus Lactobacillus, comparative genomics and phylogenomic approaches, followed by functional analyses were performed on L. rossiae DSM 15814T, showing how this type strain not only occupies an independent phylogenetic branch, but also possesses genomic features underscoring its biotechnological potential. This strain in fact represents one of a small number of bacteria known to encode a complete de novo biosynthetic pathway of vitamin B12 (in addition to other B vitamins such as folate and riboflavin). In addition, it possesses the capacity to utilize an extensive set of carbon sources, a characteristic that may contribute to environmental adaptation, perhaps enabling the strain's ability to populate different niches.

MATERIALS
Product Number
Brand
Product Description

Glutamic acid, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
(−)-Riboflavin, from Eremothecium ashbyii, ≥98%
Sigma-Aldrich
(−)-Riboflavin, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%
Sigma-Aldrich
(−)-Riboflavin, meets USP testing specifications
Supelco
Riboflavin, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
DL-Arginine, ≥95% (NT)
Supelco
Riboflavin (B2), analytical standard
Arginine, European Pharmacopoeia (EP) Reference Standard
Riboflavin for peak identification, European Pharmacopoeia (EP) Reference Standard