• Início
  • Resultados da busca
  • Evaluation and comparison of the kinetic performance of ultra-high performance liquid chromatography and high-performance liquid chromatography columns in hydrophilic interaction and reversed-phase liquid chromatography conditions.

Evaluation and comparison of the kinetic performance of ultra-high performance liquid chromatography and high-performance liquid chromatography columns in hydrophilic interaction and reversed-phase liquid chromatography conditions.

Journal of chromatography. A (2014-12-03)
Huiying Song, Erwin Adams, Gert Desmet, Deirdre Cabooter
RESUMO

An intrinsic performance comparison is made of the reduction in analysis time that can be obtained when switching from HPLC to UHPLC column formats in HILIC and reversed-phase conditions. A detailed overview of the packing characteristics of both stationary phase types is given first. It is demonstrated that HILIC columns demonstrate higher external porosity values than their reversed-phase counterparts resulting in lower flow resistance values. Column total porosity values determined from the elution time of a small marker molecule are shown to depend strongly on the composition of the mobile phase. To omit errors that might arise from an over- or underestimation of the column void time, all plate height and kinetic plot data are therefore expressed as a function of the interstitial velocity. Although only a limited number of columns are evaluated in this study, it is shown that the column efficiency of the HILIC columns is lower than that of their reversed-phase counterparts, at least for the compounds evaluated here. Despite this lower efficiency, the kinetic performance of both stationary phase types is similar, due to the much lower viscosity of the mobile phases typically used in HILIC conditions. Finally, it is demonstrated that a similar, yet slightly larger reduction in analysis time can be obtained when switching from HPLC column formats to UHPLC formats in HILIC compared to reversed-phase conditions.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free
Sigma-Aldrich
Acetic acid, glacial, ReagentPlus®, ≥99%
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
Sigma-Aldrich
Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%
Sigma-Aldrich
Tetrahydrofuran, inhibitor-free, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Acetic acid, glacial, ACS reagent, ≥99.7%
Sigma-Aldrich
Dichloromethane, suitable for HPLC, ≥99.8%, contains amylene as stabilizer
Sigma-Aldrich
Dichloromethane, puriss. p.a., ACS reagent, reag. ISO, ≥99.9% (GC)
Sigma-Aldrich
Acetonitrile, HPLC Plus, ≥99.9%
Supelco
Ammonium acetate, suitable for mass spectrometry (MS), LiChropur, eluent additive for LC-MS
Sigma-Aldrich
Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
Sigma-Aldrich
Ammonium acetate, for molecular biology, ≥98%
Sigma-Aldrich
Thiourea, ACS reagent, ≥99.0%
Sigma-Aldrich
Dichloromethane, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%
Sigma-Aldrich
Dichloromethane, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 50-150 ppm amylene as stabilizer
Sigma-Aldrich
Acetic acid, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
Sigma-Aldrich
Tetrahydrofuran, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%
Sigma-Aldrich
Acetonitrile, for HPLC, for UV, ≥99.9% (GC)
Sigma-Aldrich
Adenine, ≥99%
Sigma-Aldrich
Uracil, ≥99.0%
Sigma-Aldrich
Adenosine, ≥99%
Sigma-Aldrich
Thymidine, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Ammonium acetate, ACS reagent, ≥97%
Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
Supelco
Acetic acid, analytical standard
Sigma-Aldrich
Tetrahydrofuran, contains 250 ppm BHT as inhibitor, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.9%
Sigma-Aldrich
Thymidine, ≥99%
Sigma-Aldrich
Ammonium acetate, reagent grade, ≥98%