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  • Early detection of high oxidative activity in patients with adenomatous intestinal polyps and colorectal adenocarcinoma: myeloperoxidase and oxidized low-density lipoprotein in serum as new markers of oxidative stress in colorectal cancer.

Early detection of high oxidative activity in patients with adenomatous intestinal polyps and colorectal adenocarcinoma: myeloperoxidase and oxidized low-density lipoprotein in serum as new markers of oxidative stress in colorectal cancer.

Laboratory medicine (2015-04-29)
Jesús Crespo-Sanjuán, María D Calvo-Nieves, Beatriz Aguirre-Gervás, José Herreros-Rodríguez, Benito Velayos-Jiménez, María J Castro-Alija, María F Muñoz-Moreno, Diego Sánchez, Nuria Zamora-González, Raquel Bajo-Grañeras, Rosa M García-Centeno, María E Largo Cabrerizo, María R Bustamante, José A Garrote-Adrados
ABSTRACT

To detect whether signs of oxidative stress appear at early stages of colorectal adenocarcinoma (CRC), particularly in the polyp stage. We also aimed to evaluate the specific entities myeloperoxidase (MPO) and oxidized low-density lipoprotein (oxLDL) as novel markers of oxidation in the plasma of patients with CRC and to study the relationship between oxidative status in plasma and patient survival. We assayed serum or plasma specimens from healthy control subjects (n = 14), from patients with intestinal polyps (n = 39), and from patients with CRC (n = 128) to calculate the modified oxidative balance score (MOBS) using several serum markers (β-carotene, lycopene, vitamin A, vitamin E, MPO, and oxLDL). We also assayed the levels of C-reactive protein (CRP) and obtained lipid profiles. Finally, we studied the survival of patients in relationship to oxidative status (antioxidants and pro-oxidants) and inflammation markers, and added theses data to the lipid profile for each patient. Oxidative stress levels increased as disease stage advanced. This increase was detected early in the polyp stage, before polyps progressed to cancer, and could be measured by the increase of such new markers as MPO and oxLDL, the decrease in antioxidants, and the MOBS value. Higher levels of oxidation correlated with lower survival. The oxidation process, which can cause mutations leading to CRC, begins development in the polyp stage. This process may be detected early by monitoring serum markers such as MPO and oxLDL.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lycopene, ≥90%, from tomato
Sigma-Aldrich
(±)-α-Tocopherol, tested according to Ph. Eur.
Sigma-Aldrich
(±)-α-Tocopherol, synthetic, ≥96% (HPLC)
Supelco
Alpha Tocopherol, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Lycopene, analytical standard
Supelco
(±)-α-Tocopherol, analytical standard
Sigma-Aldrich
Lycopene, Redivivo, 10% FS, ~10% in corn oil, ≥95.0% (sum of isomers)
Sigma-Aldrich
(+)-α-Tocopherol, from vegetable oil, Type V, ~1000 IU/g
Sigma-Aldrich
(+)-α-Tocopherol, Type VI, from vegetable oil, liquid (≥0.88M based on potency, density and molecular wt.), BioReagent, suitable for insect cell culture, ≥1000 IU/g
Sigma-Aldrich
α-Tocopherol, ≥95.5%
α-Tocopherol, European Pharmacopoeia (EP) Reference Standard