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Sperm deoxyribonucleic acid damage in normozoospermic men is related to age and sperm progressive motility.

Fertility and sterility (2014-04-03)
Stephanie Belloc, Moncef Benkhalifa, Martine Cohen-Bacrie, Alain Dalleac, Edouard Amar, Armand Zini

To evaluate sperm DNA fragmentation in normozoospermic male partners of couples undergoing infertility evaluation. Retrospective cohort study. Clinical andrology laboratory. A total of 1,974 consecutive normozoospermic men selected from a larger cohort of 4,345 consecutive, nonazoospermic men presenting for infertility evaluation. None. Clinical parameters, conventional semen parameters, and sperm DNA fragmentation assessed by flow cytometry-based TUNEL assay and reported as percent sperm DNA fragmentation (%SDF). The mean (± SD) %SDF and the proportion of men with high %SDF (>30%) were significantly lower in the normozoospermic compared with the entire cohort of 4,345 evaluable infertile men (17.6% ± 10.1% vs. 20.7% ± 12.4% and 11% vs. 20%, respectively). In the group of 1,974 normozoospermic men, %SDF was positively correlated with paternal age (r = 0.17) and inversely correlated with progressive motility (r = -0.26). In the subset of normozoospermic men with sperm parameters above the 50th percentile (≥ 73 × 10(6) sperm/mL, ≥ 55% progressive motility, and ≥ 14% normal forms, World Health Organization 2010 guidelines), 5% (4 of 83) had elevated %SDF (>30%). In this large cohort of normozoospermic men presenting for infertility evaluation, DNA fragmentation level is related to sperm motility and paternal age, and 11% of these men have high levels of sperm DNA fragmentation. Furthermore, the data indicate that a nonnegligible proportion (5%) of normozoospermic men with high-normal sperm parameters may also have significant sperm DNA fragmentation.

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Product Description

Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
Formaldehyde solution, stabilized with methanol, ~37 wt. % in H2O, certified reference material
Formaldehyde solution, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
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Formaldehyde solution, for molecular biology, BioReagent, ≥36.0% in H2O (T)
Formaldehyde solution, tested according to Ph. Eur.
Formaldehyde solution, for molecular biology, 36.5-38% in H2O
Citrate Concentrated Solution, BioReagent, suitable for coagulation assays, 4 % (w/v)
Sodium Citrate, Pharmaceutical Secondary Standard; Certified Reference Material
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Fluorescein, for fluorescence, free acid
Fluorescein, European Pharmacopoeia (EP) Reference Standard