Alginate nanofibrous mats with adjustable degradation rate for regenerative medicine.

Biomacromolecules (2015-02-07)
Hadi Hajiali, José A Heredia-Guerrero, Ioannis Liakos, Athanassia Athanassiou, Elisa Mele
RESUMO

The broad utilization of electrospun scaffolds of sodium alginate in tissue engineering is strongly limited by their high solubility in aqueous environments and by the difficulty to adjust their degradation dynamics. Here, an alternative strategy to enhance the stability and to control the degradability of alginate nanofibers is described by treating them with trifluoroacetic acid for specific time intervals. It is demonstrated that, by increasing the duration of the acid treatment procedure, a lower degradation rate of the resulting fibers in buffer solutions can be achieved. Furthermore, the produced mats are free from cytotoxic compounds and are highly biocompatible. The properties conferred to the alginate nanofibrous mats by the proposed method are extremely attractive in the production of innovative biomedical devices.

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Sigma-Aldrich
N,N-Dimethylformamide, anhydrous, 99.8%
Sigma-Aldrich
Hydrochloric acid, ACS reagent, 37%
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Acetic acid, glacial, ReagentPlus®, ≥99%
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Triethylamine, ≥99.5%
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Trifluoroacetic acid, suitable for HPLC, ≥99.0%
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Trifluoroacetic acid, ReagentPlus®, 99%
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Hydrochloric acid, ACS reagent, 37%
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Sodium hydroxide, reagent grade, ≥98%, pellets (anhydrous)
Sigma-Aldrich
Acetic acid, glacial, ACS reagent, ≥99.7%
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Formic acid, reagent grade, ≥95%
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DAPI, for nucleic acid staining
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Sodium hydroxide solution, 50% in H2O
Sigma-Aldrich
Sodium hydroxide, ACS reagent, ≥97.0%, pellets
Sigma-Aldrich
Triethylamine, ≥99%
Sigma-Aldrich
N,N-Dimethylformamide, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
Sigma-Aldrich
N,N-Dimethylformamide, ACS reagent, ≥99.8%
Sigma-Aldrich
Sodium hydroxide solution, BioUltra, for molecular biology, 10 M in H2O
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Sodium hydroxide, BioXtra, ≥98% (acidimetric), pellets (anhydrous)
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Thiazolyl Blue Tetrazolium Bromide, 98%
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Hydrogen chloride solution, 4.0 M in dioxane
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Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture
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Sodium hydroxide solution, 1.0 N, BioReagent, suitable for cell culture
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Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology
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Hydrochloric acid, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., fuming, ≥37%, APHA: ≤10
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Formic acid, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥98%
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Triethylamine, puriss. p.a., ≥99.5% (GC)
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Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
Sigma-Aldrich
Formic acid, ACS reagent, ≥96%
Sigma-Aldrich
N,N-Dimethylformamide, for molecular biology, ≥99%