Merck
  • Home
  • Search Results
  • PI-PLCβ1b affects Akt activation, cyclin E expression, and caspase cleavage, promoting cell survival in pro-B-lymphoblastic cells exposed to oxidative stress.

PI-PLCβ1b affects Akt activation, cyclin E expression, and caspase cleavage, promoting cell survival in pro-B-lymphoblastic cells exposed to oxidative stress.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2015-01-01)
Manuela Piazzi, William L Blalock, Alberto Bavelloni, Irene Faenza, Mirco Raffini, Francesca Tagliavini, Lucia Manzoli, Lucio Cocco
ABSTRACT

The phosphoinositide-dependent signal transduction pathway has been implicated in the control of a variety of biologic processes, such as the regulation of cellular metabolism and homeostasis, cell proliferation and differentiation, and apoptosis. One of the key players in the regulation of inositol lipid signaling is the phospholipase Cβ1 (PI-PLCβ1), that hydrolyzes phosphatidylinositol 4,5-bisphosphate [PtIns(4,5)P2], giving rise to the second messengers inositol triphosphate and diacylglicerol. PI-PLCβ1 has been associated with the regulation of several cellular functions, some of which have not yet been fully understood. In particular, it has been reported that PI-PLCβ1 protects murine fibroblasts from oxidative stress-induced cell death. The mediators of oxidative stress, reactive oxygen species (ROS), have been shown to regulate major epigenetic processes, causing the silencing of tumor suppressors and enhancing the proliferation of leukemic cells under oxidative stress. Investigation of the interplay between ROS, PI-PLCβ1, and their signaling mediators in leukemia might therefore reveal innovative targets of pharmacological therapy in the treatment for leukemia. In this work, we demonstrate that in pro-B-lymphoblastic cells (Ba/F3), treated with H2O2, PI-PLCβ1b conferred resistance to cell death, promoting cell cycle progression and cell proliferation and influencing the expression of cyclin A and E. Interestingly, we found that, expression of PI-PLCβ1b affects the activity of caspase-3, caspase-7, and of several protein kinases induced by oxidative stress. In particular, PI-PLCβ1b expression completely abolished the phosphorylation of Erk1/2 MAP kinases, down-regulated phosphatase and tensin homolog (PTEN), and up-regulated the phosphorylation of Akt, thereby sustaining cellular proliferation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Adenosine 5′-diphosphoribose sodium salt, ≥93%
Sigma-Aldrich
Activated charcoal, suitable for cell culture, suitable for plant cell culture
Sigma-Aldrich
Sand, white quartz, ≥99.995% trace metals basis
Sigma-Aldrich
LUDOX® AS-40 colloidal silica, 40 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® HS-40 colloidal silica, 40 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® TMA colloidal silica, 34 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® TM-50 colloidal silica, 50 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® AS-30 colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® AM colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® CL-X colloidal silica, 45 wt. % suspension in H2O
Sigma-Aldrich
1-Naphthyl phosphate monosodium salt monohydrate, ≥98% (titration), powder
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, 98%
Supelco
Silica, 99.8%
Sigma-Aldrich
Silica, fumed, powder, 0.2-0.3 μm avg. part. size (aggregate)
Sigma-Aldrich
Silica, fumed, powder
Sigma-Aldrich
LUDOX® LS colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
LUDOX® HS-30 colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
Silicon dioxide, −325 mesh, 99.5% trace metals basis
Sigma-Aldrich
LUDOX® SM colloidal silica, 30 wt. % suspension in H2O
Sigma-Aldrich
Silicon dioxide, fused (granular), 4-20 mesh, 99.9% trace metals basis
Sigma-Aldrich
LUDOX® TM-40 colloidal silica, 40 wt. % suspension in H2O
Sigma-Aldrich
Silica, mesostructured, MSU-F (cellular foam)
Supelco
Glass spheres
Sigma-Aldrich
Silicon dioxide, fused (pieces), 4 mm, 99.99% trace metals basis
Sigma-Aldrich
Silica
Sigma-Aldrich
Silicon dioxide, granular, ≥99.9%
Sigma-Aldrich
Silica, nanopowder, spec. surface area 175-225 m2/g (BET), 99.8% trace metals basis
Sigma-Aldrich
Silicon dioxide, nanopowder (spherical, porous), 5-20 nm particle size (TEM), 99.5% trace metals basis
Sigma-Aldrich
Silica, mesostructured, MCM-41 type (hexagonal)