Merck

Endothelin as a local regulating factor in the bovine oviduct.

Reproduction, fertility, and development (2014-11-06)
Yuki Yamamoto, Misa Kohka, Yoshihiko Kobayashi, Izabela Woclawek-Potocka, Kiyoshi Okuda
RESUMO

Endothelin (EDN) is a possible regulating factor of oviductal motility, which is important for the transport of gametes and embryo. To clarify the factors that control the secretion of EDN in the bovine oviduct, the expression of EDNs, EDN-converting enzymes (ECEs) and EDN receptors (EDNRs) were investigated. All isoforms of EDN (EDN1-3), ECE (ECE1 and ECE2) and EDNR (EDNRA and EDNRB) were immunolocalised in the epithelial cells of the ampulla and the isthmus. EDNRs were also immunolocalised in smooth-muscle cells. The mRNA expression of EDN2 and ECE2 was higher in cultured ampullary oviductal epithelial cells than in isthmic cells. The expression of EDN1, EDN2 and ECE2 in the ampullary tissue was highest on the day of ovulation. Oestradiol-17β increased EDN2 and ECE1 expression, while progesterone increased only ECE1 expression in cultured ampullary epithelial cells. These results indicate that EDNs are produced by epithelial cells and their target site is smooth-muscle and epithelial cells, and suggest that ovarian steroids are regulators of endothelin synthesis in ampullary oviductal epithelial cells.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
N-Boc-1,3-propanediamine, ≥97.0% (GC/NT)
Sigma-Aldrich
Dulbecco′s Modified Eagle′s Medium - high glucose, HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture
Sigma-Aldrich
Insulin from bovine pancreas, Hybri-Max, powder, suitable for hybridoma
Sigma-Aldrich
Sodium selenite, BioReagent, suitable for cell culture, ≥98%
Sigma-Aldrich
Amphotericin B solubilized, powder, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid