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T cell growth factor: parameters of production and a quantitative microassay for activity.

Journal of immunology (Baltimore, Md. : 1950) (1978-06-01)
S Gillis, M M Ferm, W Ou, K A Smith
PMID307029
ABSTRACT

Several soluble factors have recently been associated with the proliferation and differentiation of thymus-derived lymphocytes. One of these factors present in medium conditioned by T cell mitogen-stimulated lymphocytes has the ability to promote the long-term culture of normal and antigen-specific cytotoxic T cells. We report a method to test for this proliferative stimulus in the form of a sensitive microassay based upon the tritiated-thymidine incorporation of continuous murine tumor-specific cytotoxic T cell lines (CTLL). The microassay requires microliter quantitites of sample fluid and is amenable to quantitative analysis. This highly reproducible, quantitative assay for T cell growth factor (TCGF) has allowed investigation as to the kinetics of TCGF generation and has revealed that T lymphocytes are required for its production. Further investigation has supported the notion that this nonspecies-specific factor is actively removed from tissue culture medium by the proliferation of either T cell mitogen-activated lymphocytes or CTLL.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Interleukin-2 human, recombinant, expressed in E. coli, ~10000 U/mL