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  • Adjacent-possible ecological niche: growth of Lactobacillus species co-cultured with Escherichia coli in a synthetic minimal medium.

Adjacent-possible ecological niche: growth of Lactobacillus species co-cultured with Escherichia coli in a synthetic minimal medium.

Scientific reports (2017-10-19)
Kouhei Mizuno, Mamiko Mizuno, Mio Yamauchi, Aya J Takemura, Veronica Medrano Romero, Kazuya Morikawa
RESUMO

In certain conditions, members of the Lactobacillus genus are auxotrophs that have fastidious requirements for growth. Notably, Lactobacillus cannot grow in M9 medium, a minimal synthetic medium used for Escherichia coli. However, we found that some Lactobacillus strains can be grown in M9 when co-cultured with E. coli K-12. In the co-culture, L. casei proliferates exponentially, reaching cell densities of 10

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Ammonium citrate tribasic, ≥97% (titration)