[Pular para o conteúdo](https://www.sigmaaldrich.com#main-content) [![Merck](https://www.sigmaaldrich.com/static/logos/purple/merck.svg)](https://www.sigmaaldrich.com/BR/pt) Produtos Carrinho0 BRPT Produtos ProdutosAplicativosServiçosRecursosSuporte [Login / Registrar-se](https://www.sigmaaldrich.com/oidc-sign-in) [Nº do Pedido](https://www.sigmaaldrich.com/BR/pt/order-lookup) [Pedido rápido](https://www.sigmaaldrich.com/BR/pt/quick-order) Carrinho0 [Home](https://www.sigmaaldrich.com/BR/en)[Sample Purification](https://www.sigmaaldrich.com/BR/en/applications/analytical-chemistry/sample-preparation/purification)Selection of Purification Equipment # Selection of Purification Equipment Simple IEX chromatography, such as elution by a step-gradient, can be performed using a syringe or peristaltic pump with prepacked HiTrap columns. A chromatography system is required in order to achieve a high resolution separation using accurately controlled linear gradient elution, to take advantage of the high flow rates of modern media, or when the same column is to be used for many runs. Way of workingPilotExplorer 100Purifier 10FPLCPrimeSyringe or peristaltic pump + HiTrap column Simple, step-gradient elution✓✓✓✓✓✓ Reproducible performance for routine separation✓✓✓✓✓ Optimization of one step separation to increase purity✓✓✓✓✓ System control and data handling for regulatory requirements, e.g. GLP✓✓✓✓ Automatic method development and optimization✓✓✓✓ Automatic buffer preparation✓✓✓ Automatic pH scouting✓✓✓ Automatic media or column scouting✓✓ Automatic multi-step purification✓✓ Scale up, process development and transfer to production✓✓ Proven sanitary design for cGMP with easily exchanged wetted parts✓ Standard ÄKTAdesign configurations ![Standard ÄKTAdesign configurations](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/analytical-chemistry/purification/akt-design-configurations.jpg "Standard ÄKTAdesign configurations") __Figure 1.__ Standard ÄKTAdesign configurations ## Materials Desculpe, ocorreu um erro inesperado Response not successful: Received status code 500 __Related Articles__ - [Bind/Elute vs Flowthrough Mode in Multimodal Chromatography](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/purification/bind-elute-vs-flowthrough-mode-in-multimodal-chromatography) - [Chiral Chromatography Frequently Asked Questions](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/purification/faq) - [FastWoRX for Faster and Greener Alternative to Liquid-Liquid Extraction](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/purification/fastworx) - [High Purity Carbon Adsorbents for Chromatography](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/purification/high-purity-carbon-adsorbents) - [Plasmid purification by ion exchange chromatography with Genopure kits](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/purification/ion-exchange-chromatography) - [LRA (Lipid Removal Agent) for Biopharmaceutical Production](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/purification/lra-lipid-removal) - [Immobilized Metal Chelate Affinity Chromatography (IMAC)](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/purification/metal-chelate-afnity-chromatography) - [Non-volatile and Volatile Buffer Systems](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/purification/non-volatile-and-volatile-buffer-systems) - [View More](https://www.sigmaaldrich.com/BR/en/search/facet-search?focus=sitecontent&term=facet-search) Topo __Faça login para continuar__ Para continuar lendo, faça login ou crie uma conta. 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