[Pular para o conteúdo](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/BR/pt) Produtos Carrinho0 BRPT Produtos ProdutosAplicativosServiçosRecursosSuporte [Login / Registrar-se](https://www.sigmaaldrich.com/oidc-sign-in) [Nº do Pedido](https://www.sigmaaldrich.com/BR/pt/order-lookup) [Pedido rápido](https://www.sigmaaldrich.com/BR/pt/quick-order) Carrinho0 [Home](https://www.sigmaaldrich.com/BR/en)[Solid Phase Microextraction (SPME)](https://www.sigmaaldrich.com/BR/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction)Introduction to SPME # Introduction to SPME [Learn More About SPME](https://www.sigmaaldrich.com/BR/en/applications/analytical-chemistry/sample-preparation/solid-phase-microextraction) Solid-phase microextraction or SPME is a unique solventless sample prep technique. SPME uses a polymer-coated fiber to concentrate volatile and semi-volatile organics in one extraction step. SPME requires no solvents or complicated extraction apparatus. The SPME fiber is attached to a plunger inside a protective needle, which is attached to a holder that looks like a micro liter syringe. The plunger inside of the holder moves the SPME fiber into and out of a protective needle for extraction of organics from a sample matrix. The organics are adsorbed onto the liquid coating on the fiber for a predetermined amount of time. The SPME fiber is retracted into the protective needle and injected into the heated injector of the gas chromatograph. The organics are then thermally transferred onto the chromatographic column for separation and measurement. Afterwards, the SPME fiber is retracted into the needle and removed from the injection port for subsequent sample analysis. __Related Articles__ - [Biodiesel](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/biodiesel) - [Automated Solid Phase Microextraction (SPME) Sampling](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/automated-spme-sampling) - [Bioanalysis with SPME](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/bioanalysis-with-spme) - [BioSPME for Plasma Protein Binding Assay: Details of Method Development and Optimization](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/biospme-plasma-protein-binding-assay) - [Solid Phase Microextraction: Frequently Asked Questions](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/faq) - [GC/MS/MS Analysis of BPA in Energy Drink](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/gc-ms-ms-analysis-of-bisphenol-a-bpa-in-a-fruit-flavored-energy-drink-cla010218a) - [Rugged SPME Sampling](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/nitinol-core-spme-fibers) - [SPME Fiber Performance Tips for Volatiles & Semi-Volatiles](https://www.sigmaaldrich.com/BR/en/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/performance-tips) - [View More](https://www.sigmaaldrich.com/BR/en/search/facet-search?focus=sitecontent&term=facet-search) Topo __Faça login para continuar__ Para continuar lendo, faça login ou crie uma conta. 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