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Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

Name: Purine Nucleoside Phosphorylase, Human, Recombinant, <i>E. coli</i>
Brand: MM

Synonym(s):

PNP

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About This Item

NACRES:

NA.42

UNSPSC Code:

12352202

Specific activity:

≥25 units/mg protein

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Segment

100

form

liquid

specific activity

≥25 units/mg protein

manufacturer/tradename

Calbiochem^®

shipped in

dry ice

storage temp.

−20°C

Description

General description

Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.

Physical form

In 50 mM Tris-HCl, pH 7.6.

Analysis Note

Single band by SDS-PAGE

Other Notes

Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.

One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P_i into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item	539735	324717	480708
Sigma-Aldrich 540221 Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli	Sigma-Aldrich 539735 PTP1B, Human, Recombinant, E. coli	Sigma-Aldrich 324717 Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli	Sigma-Aldrich 480708 α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli
description -	description -	description Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.	description -
specific activity ≥25 units/mg protein	specific activity ≥20 U/mg	specific activity ≥3 units/mL, ≥30 units/mg protein	specific activity ≥10 units/mL, ≥250 units/mg protein
form liquid	form liquid	form liquid	form liquid
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
shipped in dry ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
storage temp. −20°C	storage temp. −70°C	storage temp. 2-8°C	storage temp. 2-8°C
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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