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05-623

Sigma-Aldrich

Anti-RNA polymerase II Antibody, clone CTD4H8

clone CTD4H8, Upstate®, from mouse

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Synonym(s):
Anti-NEDHIB, Anti-POLR2, Anti-POLRA, Anti-RPB1, Anti-RPBh1, Anti-RPO2, Anti-RPOL2, Anti-RpIILS, Anti-hRPB220, Anti-hsRPB1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CTD4H8, monoclonal

species reactivity

Saccharomyces cerevisiae, human, mouse, rat

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... POLR2A(5430)
mouse ... Polr2A(20020)
rat ... Polr2A(363633)

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This Item
05-623-Z05-623-AF48805-623-AF647
species reactivity

Saccharomyces cerevisiae, human, mouse, rat

species reactivity

mouse, yeast, rat, human

species reactivity

human, mouse

species reactivity

mouse, human

clone

CTD4H8, monoclonal

clone

CTD4H8, monoclonal

clone

CTD4H8, monoclonal

clone

CTD4H8, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

ChIP: suitable (ChIP-seq), western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

immunocytochemistry: suitable

UniProt accession no.

P24928

UniProt accession no.

P24928

UniProt accession no.

P24928

UniProt accession no.

P24928

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General description

RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Specificity

This antibody recognizes Phospho- and non-phospho-RNA polymerase II.

Immunogen

Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.

Application

Anti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Routinely evaluated by western blot on HeLa nuclear extracts.

Western Blot Analysis:
0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts

Target description

210-220 kDa

Physical form

Format: Purified
Protein G Chromatography
Purified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
A431 nuclear extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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Inhibition of activator protein-1 by sulforaphane involves interaction with cysteine in the cFos DNA-binding domain: implications for chemoprevention of UVB-induced skin cancer.
Dickinson, SE; Melton, TF; Olson, ER; Zhang, J; Saboda, K; Bowden, GT
Cancer Research null
Intragenic transcription of a noncoding RNA modulates expression of ASP3 in budding yeast.
Huang, YC; Chen, HT; Teng, SC
RNA null
Correlation of dysfunction of nonmuscle myosin IIA with increased induction of Cyp1a1 in Hepa-1 cells.
Ebina M, Shibazaki M, Kudo K, Kasai S, Kikuchi H
Biochimica et Biophysica Acta null
Zfp143 regulates Nanog through modulation of Oct4 binding.
Xi Chen, Fang Fang, Yih-Cherng Liou, Huck-Hui Ng, Xi Chen, Fang Fang, Yih-Cherng Liou, Huck-Hui Ng
Stem Cells null
M Patturajan et al.
The Journal of biological chemistry, 274(39), 27823-27828 (1999-09-17)
Monoclonal antibodies that recognize specific carboxyl-terminal domain (CTD) phosphoepitopes were used to examine CTD phosphorylation in yeast cells lacking carboxyl-terminal domain kinase I (CTDK-I). We show that deletion of the kinase subunit CTK1 results in an increase in phosphorylation of

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