14-326-M

Src Protein, active, 10 µg

Active, N-Terminal His6-tagged, recombinant, full-length, human Src. For use in Kinase Assays.

• Synonym(s): SRC1
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.26

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in Sf21 cells
• form: liquid
• mol wt: M_w 61.7 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• solubility: soluble
• NCBI accession no.: NM_005417.3; NM_198291.1
• UniProt accession no.: P12931
• Gene Information: human ... SRC(6714)

Description

General description

Research area: Cell Signaling

N-Terminal His6-tagged, recombinant, full-length, human Src.

Src proteins are non-receptor protein tyrosine kinases, that belong to the Src family of kinases.[1] These proteins have a unique structure consisting of an N-terminal region with a 14-carbon myristoyl group, two Src homology domains (SH2 and SH3), a catalytic tyrosine-protein kinase domain (SH1), and a short C-terminal tail. The SH1 domain is catalytic in function, while the SH2 and SH3 domains are non-catalytic and regulatory in nature.[2]

Product Source: Human c-src, expressed in Sf21 cells.

Application

The Src protein may be used to study the interaction between CD133 and Src protein and its effect on FAK phosphorylation and cell migration through in vitro kinase assay.[3]

Biochem/physiol Actions

Src protein tyrosine kinases are involved in signaling pathways that control a plethora of cellular functions ranging from cell cycle, proliferation, differentiation, gene transcription, immune responses, apoptosis, etc. Upon its activation, Src phosphorylates and activates several other kinases Like MAPK (Mitogen-Activated Protein Kinase), p38, and ERK (Extracellular signal-regulated kinase). Activated MAPK (Mitogen-Activated Protein Kinase) regulates inflammation, cell development, differentiation and senescence. P38 is involved with the migration and survival of endothelial cells and extracellular signal-regulated kinase (ERK) promotes the proliferation and inflammation of endothelial cells.[2] They play a key role in the regulation of cytoskeletal organization through the phosphorylation of substrates like Actin filament-associated protein 1 (AFAP1) and cortactin (CTTN).[4] Src also plays a crucial role in maintaining cell adhesion, morphology, motility, and bone resorption by interacting with focal adhesion kinases.[1] Src tyrosine kinase activity is upregulated in a variety of cancers including colon, lung, and pancreatic cancer, etc.[1]

Packaging

2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-326M when ordering the 250μg size.

Quality

Routinely evaluated by phosphorylation of Src Substrate Peptide

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable