14-404-M

ZAP-70 Protein, active, 20 µg

Active, recombinant, human, full length ZAP-70 containing a C-terminal His6 tag, for use in Kinase Assays.

• Synonym(s): 70 kDa zeta-chain associated protein, SRK, Syk-related tyrosine kinase
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in Sf21 cells
• form: liquid
• shelf life: 6 mo.
• mol wt: M_w 70 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_001079.3; NM_207519.1
• UniProt accession no.: P43403
• storage temp.: −20°C
• Gene Information: human ... ZAP70(7535)

Description

General description

Research area: CELL SIGNALING

Product Source: expressed in Sf21 Recombinant, human, full length ZAP-70 containing a C-terminal His6-tag

Zeta-chain-associated protein kinase 70 (ZAP-70) is a cytoplasmic tyrosine kinase that consists of tandem SH2 (tSH2) domains at the beginning, linked with a long linker region, and a tyrosine kinase domain at the C-terminal end. [1][2]

Biochem/physiol Actions

Protein Target: ZAP-70

Target Sub-Family: TK

Zeta-chain-associated protein kinase 70 (ZAP-70) plays a vital role in the development and function of T cells and natural killer cells. [3][2] It interacts directly with the activated T-cell receptor and regulates downstream signals.[3] In addition, ZAP-70 also mediates B-cell receptor (BCR) signaling. [1] Mutations in ZAP-70 have been associated with severe combined immunodeficiency (SCID), [3] chronic lymphocytic leukemia (CLL) [1] , and the development of autoreactive T cells that attack the joints. [4]

Physical form

Ni²⁺/NTA-agarose

Preparation Note

6 months at -20C

Analysis Note

routinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1) substrate peptide

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1