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MilliporeSigma

14-777-M

Sigma-Aldrich

Plk1 Protein, active, 10 µg

Active, N-terminal 6His tagged, recombinant, human Plk1. For use in Kinase Assays.

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14-777
$727.00
14-777M
$5,870.00

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41

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biological source

human

Quality Level

100

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 69.3 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_005030

UniProt accession no.

P53350
Q07832 (mouse)
Q62673 (rat)

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14-572-M14-452-M14-650-M
Plk1 Protein, active, 10 µg Active, N-terminal 6His tagged, recombinant, human Plk1. For use in Kinase Assays.

Sigma-Aldrich

14-777-M

Plk1 Protein, active, 10 µg

PLK3 Protein, active, 10 µg Active, N-terminal His6-tagged recombinant human Plk3, residues 19-301, for use in Kinase Assays.

Sigma-Aldrich

14-572-M

PLK3 Protein, active, 10 µg

PDK1 Protein, active, 10 µg Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.

Sigma-Aldrich

14-452-M

PDK1 Protein, active, 10 µg

PKG1β Protein, active, 10 µg Active, N-terminal 6His-tagged, recombinant, full length PKG1β, for use in Kinase Assays.

Sigma-Aldrich

14-650-M

PKG1β Protein, active, 10 µg

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

biological source

human

biological source

human

biological source

human

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 69.3 kDa

mol wt

Mw 36 kDa

mol wt

Mw 59 kDa

mol wt

Mw 81.6 kDa

UniProt accession no.

P53350, Q62673 (rat), Q07832 (mouse)

UniProt accession no.

Q9H4B4

UniProt accession no.

O15530

UniProt accession no.

P14619

NCBI accession no.

NM_005030

NCBI accession no.

NM_004073.2

NCBI accession no.

NM_002613.3, NM_031268.4

NCBI accession no.

NM_001098512.1, NM_006258.2

General description

N-terminal 6His tagged, recombinant, human Plk1.
Product Source: Expressed by baculovirus in Sf21 insect cells.

Application

Research Category
Inflammation & Immunology

Biochem/physiol Actions

Protein Target: Plk1
Target Sub-Family: Misc

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-777M when ordering the 250 µg size.

Physical form

50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose

Preparation Note

Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shrividya Sana et al.
Life science alliance, 1(6), e201800223-e201800223 (2018-11-21)
Proper orientation of the mitotic spindle defines the correct division plane and is essential for accurate cell division and development. In metazoans, an evolutionarily conserved complex comprising of NuMA/LGN/Gαi regulates proper orientation of the mitotic spindle by orchestrating cortical dynein

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