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Chromolith® WP 300 Protein A

L × I.D. 25 mm × 2 mm HPLC column

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Synonym(s):
Chromolith® WP 300 Protein A 25-2 HPLC column

Quality Level

feature

endcapped: no

parameter

2-45 °C temp. range
200 bar pressure

technique(s)

HPLC: suitable

column L × I.D.

25 mm × 2 mm

matrix

monolithic silica

matrix active group

Protein A bonding phase

pore size

2 μm macroporosity
30 nm mesoporosity

operating pH

1.5-7.5

storage temp.

2-8°C

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General description

Affinity chromatography is a selective technique that takes advantage of specific molecular interactions, for example, antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith® WP 300 Protein A, 25-2 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
  • Rapid and accurate titer determination across a wide range of injection volumes
  • Consistent binding efficiencies even at high flow rates
  • Expanded column lifetimes


Learn more:
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Method development for titer determination of three different monoclonal antibodies - cetuximab, trastuzumab, and universal antibody standard using a Chromolith® WP 300 Protein A column.

Related Content

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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