171539
AMPK (α2/β1/γ1), His•Tag®, Human, Recombinant,S. frugiperda
Synonym(s):
AMP Kinase (α2/β1/γ1)
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5 μG
$973.00
About This Item
UNSPSC Code:
12352203
NACRES:
NA.77
Recommended Products
Assay
≥75% (SDS-PAGE)
Quality Level
form
liquid
specific activity
≥270 nmol/min-mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Full length, recombinant, human AMPK consisting of subunits α2/β1/γ1, each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The α-subunit contains a kinase domain, the β-subunit contains a glycogen binding domain, and the γ-subunit contains the regulatory AMP binding domain. The active complex is useful in the study of kinase activity regulation and inhibitor screening.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
Kinase activity was measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP.
Physical form
In 150 mM NaCl, 50 mM Tris-HCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.
Reconstitution
Following reconstitution, aliquot and freeze (-70°C).
Other Notes
Foretz, M., et al. 2005. Diabetes54, 1331.
Viollet, B., et al. 2003. Biochem. Soc. Trans.31, 216.
Viollet, B., et al. 2003. Biochem. Soc. Trans.31, 216.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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Activation of AMP-activated protein kinase (AMPK) through inhibiting interaction with prohibitins.
Kanagaki, et al.
iScience, 26, 106293-106293 (2023)
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