197220

Bcl-2 Binding Peptide, Cell-Permeable

A cell-permeable peptide that binds to Bcl-2 with high affinity (IC₅₀ = 130 nM in a competition binding study) and induces apoptosis.

• Synonym(s): Bcl-2 Binding Peptide, Cell-Permeable, cpm-1285, Decanoyl-KN¹⁴⁰LWAAQRYGRELRRMSDEFEGSFKGL¹⁶⁵-OH
• Empirical Formula (Hill Notation): C₁₅₃H₂₄₀N₄₄O₄₂S
• Molecular Weight: 3399.88
• UNSPSC Code: 12352202

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: off-white
• solubility: DMSO: 10 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A cell-permeable Bcl-2 binding peptide that induces apoptosis in Bcl-2 expressing cells with no apparent effect on normal peripheral lymphocytes. Also reported to diminish the growth of human myeloid leukemia in severe combined immunodeficient mice. The peptide is derived from the BH3 domain (residues 140-165, a death domain) of Bad and is modified to be cell-permeable by attaching a decanoyl moiety at the N-terminus.

A cell-permeable peptide that binds to Bcl-2 with high affinity (IC₅₀ = 130 nM in a competition binding study) and induces apoptosis. Displays antitumor activity in cells overexpressing Bcl-2 with no apparent effect on normal peripheral lymphocytes. The peptide is derived from the BH3 domain (residues 140 - 165, a “death domain”) of Bad and is modified to be cell permeant by attaching a decanoyl moiety at the N-terminus.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Bcl-2

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 130 nM against Bcl-2

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

CH₃(CH₂)₈CONH-Lys-Asn-Leu-Trp-Ala-Ala-Gln-Arg-Tyr-Gly-Arg-Glu-Leu-Arg-Arg-Met-Ser-Asp-Glu-Phe-Glu-Gly-Ser-Phe-Lys-Gly-Leu-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 weeks at -20°C.

Other Notes

Wang, J-L., et al. 2000. Cancer Res.60, 1498.
Kelekar, A., et al. 1997. Mol. Cell. Biol.17, 7040.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable