219498

Cathepsin S Substrate, Fluorogenic

An internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS, but not by Cathepsin B & L.

• Synonym(s): Cathepsin S Substrate, Fluorogenic, Mca-GRWPPMG~LPWEK(Dnp)- D-R-NH2, Mca-GRWPPMG~LPWEK(Dnp)-D-R-NH2
• Empirical Formula (Hill Notation): C₉₃H₁₂₃N₂₅O₂₃S
• Molecular Weight: 1991.19
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: yellow
• solubility: DMSO: 5 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

An internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS (k_cat/K_m = 1.018 µM^-1 S^-1), but not by Cathepsin B & L (k_cat/K_m = 0.004 and 0.032 µM^-1 S^-1, respectively). Studies using CatS-specific inhibitor LHVS reveal that the substrate is not cleaved by other proteolytic enzymes, including CatB, CatH, CatL, and CatX, present in endosomal extracts from human B cell line WT100, dendritic cells, and macrophages (excitation maximum: 340 nm; emission maximum: 405 nm).

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Mca-Gly-Arg-Trp-Pro-Pro-Met-Gly~Leu-Pro-Trp-Glu-Lys(Dnp)-D-Arg-NH₂

Other Notes

Lutzner, N., and Kalbacher, H. 2008. J. Biol. Chem.283, 36185.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable