conjugate
peroxidase conjugate
Quality Level
form
solid
specific activity
≥125 purpurogallin units/mg protein (HRP)
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
shipped in
ambient
storage temp.
2-8°C
General description
Sold on the basis of HRP content.
Suitable for the demonstration of dendritic branching in retrogradely labeled neurons. Has been shown to be up to 50 times more sensitive than free peroxidase as both an orthogradely and retrogradely transported marker in the rat visual system.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Demonstration of dendritic branching in retrogradely labeled neurons
Demonstration of dendritic branching in retrogradely labeled neurons
Product does not compete with ATP.
Reversible: no
Packaging
yes
Warning
Toxicity: Harmful (C)
Physical form
Lyophilized from 10 mM sodium phosphate buffer, pH 7.5.
Preparation Note
Prepared by a modification of the two-step conjugation method of Avrameas, S., and Ternynck, T. 1971. Chromatographically purified and lyophilized.
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Reconstitute in ≤100 µl of sterile distilled H₂O.
Other Notes
Raappana, P., and Arvidsson, J. 1993. J. Comp. Neurol. 328, 103.
Trojanowski, J.Q. 1983. J. Neurosci. Methods9, 185.
Trojanowski, J.Q., et al. 1982. Brain Res.231, 33.
Wan, X.S.T., et al. 1982. Exp. Neurol.78, 167.
Avrameas, S., and Ternynck, T. 1971. Immunochemistry8, 1175.
Trojanowski, J.Q. 1983. J. Neurosci. Methods9, 185.
Trojanowski, J.Q., et al. 1982. Brain Res.231, 33.
Wan, X.S.T., et al. 1982. Exp. Neurol.78, 167.
Avrameas, S., and Ternynck, T. 1971. Immunochemistry8, 1175.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Resp. Sens. 1
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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