234567M

Concanavalin A, Canavalia ensiformis

Concanavalin A, Canavalia ensiformis, CAS 11028-71-0, is a carbohydrate-binding protein that agglutinates various animal cells. Restores the growth pattern of tumor cells to that of normal cells.

• Synonym(s): Concanavalin A, Canavalia ensiformis, Con A, Jack Bean Hemagglutinin
• CAS Number: 11028-71-0
• MDL number: MFCD00071069
• UNSPSC Code: 12352202
• NACRES: NA.42

Properties

• Quality Level: 100
• description: Merck USA index - 14, 2495
• form: lyophilized
• potency: ≤20 μg/mL lectin agglutination activity (as determined from serial dilutions of a 1 mg/mL solution)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• pI: 4.5 - 5.5
• storage temp.: 2-8°C

Description

General description

Carbohydrate-binding protein that agglutinates various animal cells. Also known to restore the growth pattern of tumor cells to that of normal cells. T lymphocyte activator. Has binding sites for metal ions, α-D-mannose, and α-D-glucose. Has a pI of 4.5-5.5.

Warning

Toxicity: Harmful (C)

Preparation Note

To dissolve, add Con A slowly to a solution of 10 mM HEPES buffered saline, 100 µM CaCl₂, pH 8.5. Allow the lectin to dissolve without agitation.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) or add 0.08% NaN₃ and refrigerate (4°C). Stock solutions are stable for up to 6 months at -20°C or for up to 3 months at 4°C.

Analysis Note

The agglutination activity is the lowest conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25°C; requires type A human blood.

Other Notes

Gantner, F., et al. 1995. Hepatology 21, 190.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334,H361
• Precautionary Statements: P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable