260913

DNase I, Bovine Pancreas

Native DNase I from bovine pancreas.

• Synonym(s): DNase I, Bovine Pancreas, Deoxyribonuclease I
• CAS Number: 9003-98-9
• Enzyme Commission number: 3.1.21.1 (BRENDA, IUBMB)
• MDL number: MFCD00130918

Properties

• biological source: bovine pancreas
• form: lyophilized
• specific activity: ≥60,000 dornase units/mg dry wt
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• solubility: sodium acetate buffer: soluble; water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units

Note: 32 Dornase units equal ~1 Kunitz unit; 1 MU = 1,000,000 Dornase units.

Packaging

10 mu in Glass bottle

25 , 210, 290, 400 mu

Warning

Toxicity: Standard Handling (A)

Unit Definition

One Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun.269, 481.
Jones, S.J., et al. 1996. J. Mol. Biol.264, 1154.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable