346101-M

Androgen Receptor, His•Tag^®, Human, Recombinant, S. frugiperda

recombinant human, expressed in S. frugiperda

• Synonym(s): AR, Human, Recombinant, Dihydrotestosterone Receptor, Human, Recombinant
• UNSPSC Code: 12352202

Properties

• biological source: human
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• storage temp.: −70°C
• Gene Information: human ... AR(367)

Description

General description

Research area: CELL CYCLE

Recombinant, human androgen receptor (AR) fused at the N-terminus to a Flag-Tag sequence and at the C-terminus to His•Tag sequence, expressed in S. frugiperda insect cells expressed using a baculovirus expression system, and purified by affinity chromatography. It is located at X chromosome and is expressed in various tissues. It consists of three primary functional domains: the N-terminal transcriptional regulation domain, the DNA binding domain (DBD), and the ligand binding domain.[1]

Biochem/physiol Actions

The androgen receptor (AR) has significant biological effects on bone, muscle, prostate, adipose tissue, as well as on the reproductive, cardiovascular, immune, neural, and haemopoietic systems.[1]

The activity of androgen is mediated by the binding of the intracellular androgen receptor which, when activated, translocates to the nucleus and modulates specific gene transcription by interaction with cis-acting hormone response elements in the target genes.

Warning

Toxicity: Standard Handling (A)

Unit Definition

1.0 unit is equivalent to 1 ng protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.

One unit is defined as 1 ng purified protein.

Physical form

In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Chen, G., et al. 2005. Prostate63, 395.
Estebanez-Perpina, E., et al. 2005, J. Biol. Chem.280, 8060.
Gao, S., et al. 2005. Mol. Endocrinol.19, 1792.
Hillmer, A.M., et al. 2005. Am. J. Hum. Genet.77, 140.
Kinoshita, M., et al. 2005, Int. J. Urol.12, 390.
Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A.102, 5156;
Marques, R.B., et al. 2005. Int. J. Cancer117, 221;
Metzger, E., et al. 2005. Nature437, 436;
Murthy, S., et al. 2005. Prostate64, 362;
Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA89, 5946.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1