371958

H-8, Dihydrochloride

A potent, cell-permeable, reversible, and ATP-competitive inhibitor of cyclic-nucleotide-dependent protein kinases.

• Synonym(s): H-8, Dihydrochloride, N-[2-(Methylamino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor I
• Empirical Formula (Hill Notation): C₁₂H₁₅N₃O₂S · 2HCl
• CAS Number: 113276-94-1
• Molecular Weight: 338.25
• MDL number: MFCD00036962
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: >95% (TLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: 10 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C12H15N3O2S.2ClH/c1-13-7-8-15-18(16,17)12-4-2-3-10-9-14-6-5-11(10)12;;/h2-6,9,13,15H,7-8H2,1H3;2*1H
• InChI key: RJJLZYZEVNCZIW-UHFFFAOYSA-N

Description

General description

A potent, cell-permeable, reversible, and ATP-competitive, and highly active inhibitor of cyclic-nucleotide-dependent protein kinases. Inhibits myosin light chain kinase (K_i = 68 µM), protein kinase A (K_i = 1.2 µM), protein kinase C (K_i = 15 µM), and protein kinase G (K_i = 480 nM).

Biochem/physiol Actions

Cell permeable: yes

Product competes with ATP.

Reversible: yes

Target K_i: 68 µM against MLCK

Warning

Toxicity: Standard Handling (A)

Preparation Note

Further dilute with aqueous buffer just prior to use.

Other Notes

Ido, M., et al. 1991. Br. J. Cancer 64, 1103.
Hagiwara, M., et al. 1987. Mol. Pharmacol. 31, 523.
Inagaki, M., et al. 1986. Mol. Pharmacol.29, 577.
Hidaka, H., et al. 1984. Biochemistry23, 5036.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable